Human IL-10 ELISA Kit | IL10 elisa kit

Human IL-10 PicoKine ELISA Kit

Cat. Num. AAA45330

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Reactivity: Human
• Synonyms: IL-10; N/A; Human IL-10 PicoKine ELISA Kit; Interleukin-10; interleukin 10; CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL 10; IL10; IL10_HUMAN; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; T cell growth inhibitory factor; TGIF; IL10 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human HSPG2
• Sequence Length: 178
• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
• Detection Range: 0.624ng/ml-40ng/ml
• Sensitivity: <0.05ng/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

Related Product Information for IL10 elisa kit

Principle of the Assay: The Picokine Human HSPG2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human HSPG2 with a 96-well strip plate that is pre-coated with antibody specific for HSPG2. The detection antibody is a biotinylated antibody specific for HSPG2. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human HSPG2 with immunogen: Expression system for standard: NSO, Immunogen sequence: E3687-S4391. The kit is analytically validated with ready to use reagents. To measure Human HSPG2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human HSPG2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human HSPG2 in the sample!!Background/Introduction: Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.

NCBI and Uniprot Product Information

• NCBI GI #: 124292
• NCBI GeneID: 3586
• UniProt Accession #: P22301
• Molecular Weight: 20,517 Da
• NCBI Official Full Name: Interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• UniProt Gene Name: IL10
• UniProt Synonym Gene Names: IL-10; CSIF
• UniProt Entry Name: IL10_HUMAN