Standard Curve (Sample)
General IL-17A total antigen assay ELISA Kit | IL17A elisa kit
Mouse IL-17A total antigen assay ELISA Kit
Gene Names
Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
Reactivity
General
Synonyms
IL-17A total antigen assay; N/A; Mouse IL-17A total antigen assay ELISA Kit; IL17A elisa kit
Reactivity
General
Form/Format
Complete Kit
Sequence Length
158
Samples
Cell culture media
Sensitivity
The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.056-0.069) and calculating the corresponding concentration. The MDD was 4.8 pg/ml.
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in ten independent assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL17A elisa kit
Intended Uses: This mouse IL-17A antigen assay is intended for the quantitative determination of total mouse IL-17A antigen in cell culture media. For research use only.
Principle of the Assay: Mouse IL-17A will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse IL-17A. The amount of color development is proportional to the concentration of mouse IL-17A in the samples.
Background: Mouse Interleukin-17A (aka IL-17, IL-17A or CTLA-8) is a 133 amino acid disulfide-linked homodimeric glycoprotein that is the founding member of the IL-17 family of proteins [1-2]. IL-17A is a proinflammatory cytokine that participates in neutrophil recruitment and is primarily expressed in CD4+ T cells [3]. IL-17A has been shown in a mouse knockout model to play a vital role in allergen-specific immune responses via T cell activation [4]. It binds to the IL-17RA and IL-17RC receptors which are expressed in TH17, CD8+ T, gammadeltaT, NK, NKT and LTi cells [5].
Principle of the Assay: Mouse IL-17A will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse IL-17A. The amount of color development is proportional to the concentration of mouse IL-17A in the samples.
Background: Mouse Interleukin-17A (aka IL-17, IL-17A or CTLA-8) is a 133 amino acid disulfide-linked homodimeric glycoprotein that is the founding member of the IL-17 family of proteins [1-2]. IL-17A is a proinflammatory cytokine that participates in neutrophil recruitment and is primarily expressed in CD4+ T cells [3]. IL-17A has been shown in a mouse knockout model to play a vital role in allergen-specific immune responses via T cell activation [4]. It binds to the IL-17RA and IL-17RC receptors which are expressed in TH17, CD8+ T, gammadeltaT, NK, NKT and LTi cells [5].
Product Categories/Family for IL17A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,490 Da
NCBI Official Full Name
interleukin-17A
NCBI Official Synonym Full Names
interleukin 17A
NCBI Official Symbol
Il17a
NCBI Official Synonym Symbols
Il17; Ctla8; IL-17; Ctla-8; IL-17A
NCBI Protein Information
interleukin-17A; cytotoxic T-lymphocyte-associated antigen 8
UniProt Protein Name
Interleukin-17A
UniProt Gene Name
Il17a
UniProt Synonym Gene Names
Ctla8; Il17; IL-17; IL-17A; CTLA-8
UniProt Entry Name
IL17_MOUSE
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Product Notes
The General IL17A il17a (Catalog #AAA37774) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA37774 ELISA Kit recognizes General IL17A. It is sometimes possible for the material contained within the vial of "IL-17A total antigen assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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