Standard Curve (Sample)
Human IL-1 alpha ELISA Kit | IL1A elisa kit
Human IL-1 alpha PicoKine ELISA Kit
Gene Names
IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
Reactivity
Human
Synonyms
IL-1 alpha; N/A; Human IL-1 alpha PicoKine ELISA Kit; Interleukin-1 alpha; interleukin 1, alpha; Hematopoietin 1; IL 1 alpha; Il 1a; IL1; IL1 ALPHA; IL1A; IL1F1; Ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha; OTTHUMP00000203692; OTTMUSP00000016430; Preinterleukin 1; Pro interleukin 1; Pro interleukin 1 alpha; Prointerleukin 1; RP23-160G19.8; IL1A elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human IL1A
Sequence Length
271
Samples
Cell culture supernates, serum and plasma(heparin, edta, citrate)
Detection Range
4.7pg/ml-300pg/ml
Sensitivity
<0.5pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL1A elisa kit
Principle of the Assay: The Picokine™ Human IL1A Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL1A with a 96-well strip plate that is pre-coated with antibody specific for IL1A. The detection antibody is a biotinylated antibody specific for IL1A. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human IL1A with immunogen: Expression system for standard: E.coli, Immunogen sequence: S113-A271. The kit is analytically validated with ready to use reagents. To measure Human IL1A, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human IL1A in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL1A in the sample.
Background/Introduction: Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.
Background/Introduction: Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,607 Da
NCBI Official Full Name
Interleukin-1 alpha
NCBI Official Synonym Full Names
interleukin 1, alpha
NCBI Official Symbol
IL1A
NCBI Official Synonym Symbols
IL1; IL-1A; IL1F1; IL1-ALPHA
NCBI Protein Information
interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
UniProt Protein Name
Interleukin-1 alpha
UniProt Gene Name
IL1A
UniProt Synonym Gene Names
IL1F1; IL-1 alpha
UniProt Entry Name
IL1A_HUMAN
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Product Notes
The Human IL1A il1a (Catalog #AAA11565) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11565 ELISA Kit recognizes Human IL1A. It is sometimes possible for the material contained within the vial of "IL-1 alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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