Mouse IL-23 ELISA Kit | IL23A elisa kit

Mouse IL-23 PicoKine ELISA Kit

Cat. Num. AAA45274

• Gene Names: Il23a; p19; IL-23
• Reactivity: Mouse
• Synonyms: IL-23; N/A; Mouse IL-23 PicoKine ELISA Kit; Interleukin-23 subunit alpha; interleukin 23, alpha subunit p19; IL 23; IL 23A; IL-23 subunit alpha; IL-23-A; IL-23p19; Il23a; IL23A_HUMAN; IL23P19; Interleukin 23 alpha subunit p19; Interleukin-23 subunit p19; JKA3 induced upon T cell activation; P19; SGRF; IL23A elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse IL-23
• Sequence Length: 196
• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 2pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Mouse IL-23 PicoKine ELISA Kit standard curve)

Related Product Information for IL23A elisa kit

Principle of the assay: mouse IL-23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-23 has been precoated onto 96-well plates. Standards(sf21, (M23-S335)+(L20-A196)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-23 amount of sample captured in plate.
Background: Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. The International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812).

NCBI and Uniprot Product Information

• NCBI GI #: 81881842
• NCBI GeneID: 83430
• UniProt Accession #: Q9EQ14
• Molecular Weight: 22,071 Da
• NCBI Official Full Name: Interleukin-23 subunit alpha
• NCBI Official Synonym Full Names: interleukin 23, alpha subunit p19
• NCBI Official Symbol: Il23a
• NCBI Official Synonym Symbols: p19; IL-23
• NCBI Protein Information: interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin-23 subunit p19
• UniProt Protein Name: Interleukin-23 subunit alpha
• UniProt Gene Name: Il23a
• UniProt Synonym Gene Names: IL-23 subunit alpha; IL-23-A; IL-23p19
• UniProt Entry Name: IL23A_MOUSE