Standard Curve (Sample)
Human CD25 ELISA Kit | IL2RA elisa kit
Human CD25 ELISA Kit
Gene Names
IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Reactivity
Human
Applications
ELISA
Synonyms
CD25; N/A; Human CD25 ELISA Kit; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; IL2RA elisa kit
Reactivity
Human
Specificity
The Human CD25 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD25 proteins within the range of 31.3 pg/ml - 2000 pg/ml.
Sequence Length
272
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
15 pg/ml.
Applicable Applications for IL2RA elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL2RA elisa kit
Principle of the Assay||The Human CD25 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD25 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD25 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD25 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD25 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD25 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
Background/Introduction: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,819 Da
NCBI Official Full Name
Interleukin-2 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 2 receptor subunit alpha
NCBI Official Symbol
IL2RA
NCBI Official Synonym Symbols
p55; CD25; IL2R; IMD41; TCGFR; IDDM10
NCBI Protein Information
interleukin-2 receptor subunit alpha
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Gene Name
IL2RA
UniProt Synonym Gene Names
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
UniProt Entry Name
IL2RA_HUMAN
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Product Notes
The Human IL2RA il2ra (Catalog #AAA261706) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261706 ELISA Kit recognizes Human IL2RA. AAA Biotech's CD25 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the IL2RA il2ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD25, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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