Application Data
(Inhibition of FGF10-induced proliferation of 4MBr-5 cells by recombinant human soluble FGFR-2(IIIb)/Fc. sFGFR-2(IIIc)/Fc which do not bind FGF10 show no effect.)
FGFR-2(IIIb)/Fc Chimera Active Protein | FGFR-2 active protein
Recombinant Human Soluble FGFR-2(IIIb)Fc Chimera
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Purity
> 90% by SDS-PAGE & silver stain
Synonyms
FGFR-2(IIIb)/Fc Chimera; N/A; Recombinant Human Soluble FGFR-2(IIIb)Fc Chimera; Fibroblast growth factor receptor 2, BFR-1, FGFR2IIIb, KGFR; FGFR-2 active protein
Host
Insect cells
Purity/Purification
> 90% by SDS-PAGE & silver stain
Form/Format
Lyophilized
Sequence
RPSFSLVEDTTLEPEEPPTKYQISQPEVYVAAPGESLEVRCLLKDAAVISWTKDGVHLGPNNRTVLIGEYLQIKGATPRDSGLYACTASRTVDSETWYFMVNVTDAISSGDDEDDTDGAEDFVSENSNKRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCVVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFCKVYSDAQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTEADAGEYICKVSNYIGQANQSAWLTVLPKQQAPGREKEITASPDYLEDPRRASIEGRGDPEEPKSCDKTHTCPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Sequence Length
603
Buffer
PBS
Stabilizer
None
Reconstitution
The lyophilized sFGFR-2(IIIb)/Fc is soluble in water and most aqueous buffers and should be reconstituted to a concentration not lower than 50?g/ml.
Biological Activity
(1) The activity of sFGFR-2(IIIb)/Fc was determined by its ability to inhibit the FGF10-induced proliferation of 4MBr-5 cells. (2) Measured by its binding ability in a functional ELISA. Recombinant human soluble FGFR-2(IIIb)/Fc Chimera bind to immobilized recombinant human FGF-10 (MBS692030).
Preparation and Storage
Lyophilized samples are stable for greater than six months at –20 degree C to –70 degree C. Reconstituted sFGFR-2(IIIb)/Fc should be stored in working aliquots at -20 degree C.
Application Data
(Inhibition of FGF10-induced proliferation of 4MBr-5 cells by recombinant human soluble FGFR-2(IIIb)/Fc. sFGFR-2(IIIc)/Fc which do not bind FGF10 show no effect.)
Application Data
(Inhibition of FGF10-induced proliferation of 4MBr-5 cells by recombinant human soluble FGFR-2(IIIb)/Fc. sFGFR-2(IIIc)/Fc which do not bind FGF10 show no effect.)
Application Data
(Functional ELISA with recombinant human sFGFR-2(IIIb)/Fc Chimera. Recombinant human FGF-10 was coated at 2ug/ml and sFGFR2(IIIb)/Fc and sFGFR2(IIIc)/Fc were added [1ug/ml each]. FGF-10 binds only to FGFR2(IIIb) and not to FGFR2(IIIc).)
Application Data
(Functional ELISA with recombinant human sFGFR-2(IIIb)/Fc Chimera. Recombinant human FGF-10 was coated at 2ug/ml and sFGFR2(IIIb)/Fc and sFGFR2(IIIc)/Fc were added [1ug/ml each]. FGF-10 binds only to FGFR2(IIIb) and not to FGFR2(IIIc).)
SDS-PAGE
(SDS-PAGE analysis of recombinant human soluble FGFR-2(IIIb)/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.)
SDS-PAGE
(SDS-PAGE analysis of recombinant human soluble FGFR-2(IIIb)/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.)
Related Product Information for FGFR-2 active protein
Background: Recombinant human soluble FGFR-2 (IIIb) was fused via a Xa cleavage site with the Fc part of human IgG1. Human recombinant soluble FGFR-2 (IIIb) is a disulfide-linked homodimeric protein. In the reduced form the glycosylated subunits of sFGFR-2 (IIIb)/human Fc chimera display a molecular mass of about 90 kDa. Fibroblast Growth Factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorigenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGFR-1 to -4 are known. Multiple forms of FGFR-1 to -3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGFR-1 and -2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the ß isoform. Only the alpha isoform has been identified for FGFR-3 and FGFR-4. Additional splicing events for FGFR-1 to -3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGFR-1. Mutations in FGFR-1 to -3 have been found in patients with birth defects involving craniosynostosis.
Product Categories/Family for FGFR-2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~90 kDa
NCBI Official Full Name
fibroblast growth factor receptor 2 isoform 1
NCBI Official Synonym Full Names
fibroblast growth factor receptor 2
NCBI Official Symbol
FGFR2
NCBI Official Synonym Symbols
BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
NCBI Protein Information
fibroblast growth factor receptor 2
UniProt Protein Name
Fibroblast growth factor receptor 2
UniProt Gene Name
FGFR2
UniProt Synonym Gene Names
BEK; KGFR; KSAM; FGFR-2; KGFR
UniProt Entry Name
FGFR2_HUMAN
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Product Notes
The FGFR-2 fgfr2 (Catalog #AAA79321) is an Active Protein produced from Insect cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: RPSFSLVEDT TLEPEEPPTK YQISQPEVYV AAPGESLEVR CLLKDAAVIS WTKDGVHLGP NNRTVLIGEY LQIKGATPRD SGLYACTASR TVDSETWYFM VNVTDAISSG DDEDDTDGAE DFVSENSNKR APYWTNTEKM EKRLHAVPAA NTVKFRCPAG GNPMPTMRWL KNGKEFKQEH RIGGYKVRNQ HWSLIMESVV PSDKGNYTCV VENEYGSINH TYHLDVVERS PHRPILQAGL PANASTVVGG DVEFCKVYSD AQPHIQWIKH VEKNGSKYGP DGLPYLKVLK HSGINSSNAE VLALFNVTEA DAGEYICKVS NYIGQANQSA WLTVLPKQQA PGREKEITAS PDYLEDPRRA SIEGRGDPEE PKSCDKTHTC PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK. It is sometimes possible for the material contained within the vial of "FGFR-2(IIIb)/Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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