IHC (Immunohistochemisry)
(Immunohistochemical analysis of AP22386a on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Rabbit FGFR Polyclonal Antibody | anti-FGFR antibody
FGFR (Y766) Antibody
Gene Names
FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; bFGF-R-1
Reactivity
Human, Mouse, RatPredicted Reactivity: Chicken, Zebrafish
Applications
Western Blot, Immunohistochemistry
Synonyms
FGFR, Antibody; FGFR (Y766) Antibody; Purified Rabbit Polyclonal Antibody (Pab); Fibroblast growth factor receptor 1; FGFR-1; 2.7.10.1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331; FGFR1; CEK; FGFBR; FLG; FLT2; HBGFR; anti-FGFR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Chicken, Zebrafish
Predicted Reactivity: Chicken, Zebrafish
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB61429
Specificity
This FGFR(Y766) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 741-770 amino acids from the human region of human FGFR(Y766).
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-FGFR antibody
WB (Western Blot), IHC (Immunohistochemistry)
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemical analysis of AP22386a on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of AP22386a on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of AP22386a on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of AP22386a on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
WB (Western Blot)
(All lanes : Anti-FGFR(Y766) Antibody at 1:1000 dilutionLane 1: U-87MG whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: U-251 MG whole cell lysateLane 5: Mouse brain tissue lysateLane 6: Neuro-2a whole cell lysateLane 7: Rat brain tissue lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 92 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-FGFR(Y766) Antibody at 1:1000 dilutionLane 1: U-87MG whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: U-251 MG whole cell lysateLane 5: Mouse brain tissue lysateLane 6: Neuro-2a whole cell lysateLane 7: Rat brain tissue lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 92 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-FGFR antibody
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
Product Categories/Family for anti-FGFR antibody
References
Itoh N.,et al.Biochem. Biophys. Res. Commun. 169:680-685(1990).Dionne C.A.,et al.EMBO J. 9:2685-2692(1990).Johnson D.E.,et al.Mol. Cell. Biol. 10:4728-4736(1990).Isacchi A.,et al.Nucleic Acids Res. 18:1906-1906(1990).Wennstroem S.,et al.Growth Factors 4:197-208(1991).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,868 Da
NCBI Official Full Name
fibroblast growth factor receptor 1 isoform 10
NCBI Official Synonym Full Names
fibroblast growth factor receptor 1
NCBI Official Symbol
FGFR1
NCBI Official Synonym Symbols
CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; bFGF-R-1
NCBI Protein Information
fibroblast growth factor receptor 1; FGFR1/PLAG1 fusion; proto-oncogene c-Fgr; FMS-like tyrosine kinase 2; hydroxyaryl-protein kinase; fms-related tyrosine kinase 2; heparin-binding growth factor receptor; basic fibroblast growth factor receptor 1
UniProt Protein Name
Fibroblast growth factor receptor 1
UniProt Gene Name
FGFR1
UniProt Synonym Gene Names
BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; FGFR-1; BFGFR; bFGF-R-1; FLT-2
UniProt Entry Name
FGFR1_HUMAN
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Product Notes
The FGFR fgfr1 (Catalog #AAA290973) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FGFR (Y766) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Chicken, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's FGFR can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the FGFR fgfr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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