Human Integrin alpha-4 ELISA Kit | ITGA4 elisa kit

Human Integrin alpha-4 ELISA Kit

Cat. Num. AAA98531

• Gene Names: ITGA4; IA4; CD49D
• Reactivity: Human
• Synonyms: Integrin alpha-4; N/A; Human Integrin alpha-4 ELISA Kit; ITGA4/Integrin alpha 4/CD49d/VLA-4/269C wild type/CD49 antigen-like family member D/CD49d antigen/IA4/integrin alpha-4/integrin alpha-4 subunit/Integrin alpha-IV/very late activation protein 4 receptor; alpha 4 subunit/VLA-4 subunit alpha; ITGA4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITG alphaVbeta3. No significant cross-reactivity or interference between ITG alphaVbeta3 and analogues was observed.
• Sequence Length: 1032
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITG alphaVbeta3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITG alphaVbeta3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for ITGA4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ITG alpha Vbeta3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ITG alphaVbeta 3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ITG alphaVbeta3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ITG alphaV beta3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 67191027
• NCBI GeneID: 3676
• NCBI Accession #: NP_000876.3
• NCBI GenBank Nucleotide #: NM_000885.5
• Molecular Weight: 21,536 Da
• NCBI Official Full Name: integrin alpha-4 isoform 1 preproprotein
• NCBI Official Synonym Full Names: integrin subunit alpha 4
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4
• UniProt Protein Name: Integrin alpha-4
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D
• UniProt Entry Name: ITA4_HUMAN