WB (Western Blot)
(Recombinant KAT2A (GCN5) protein activity assay0.5 ug Histone H3.1 (Cat. No. please inquire) was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) GCN5 catalytic domain in 30 ul reaction system for 2 hours at room temperature. Products were detected by Western blot. H3ac (pan-acetyl) antibody (Cat. No. please inquire) was used to recognize acetylated histone H3.)
KAT2A recombinant protein
Recombinant KAT2A (GCN5) protein
Gene Names
KAT2A; GCN5; hGCN5; GCN5L2; PCAF-b
Synonyms
KAT2A; N/A; Recombinant KAT2A (GCN5) protein; Lysine Acetyltransferase 2A; Histone Acetyltransferase GCN5; GCN5L2; PCAF-b; KAT2A recombinant protein
Host
Baculovirus
Form/Format
Recombinant GCN5 protein is supplied at a concentration of 0.75ug/ul in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant KAT2A (GCN5) protein was expressed in a Baculovirus expression system as the full length protein (accession number NP_066564.2) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 95.6 kDa.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on Dry Ice.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. This product is for research use only and is not for use in diagnostic procedures.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. This product is for research use only and is not for use in diagnostic procedures.
WB (Western Blot)
(Recombinant KAT2A (GCN5) protein activity assay0.5 ug Histone H3.1 (Cat. No. please inquire) was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) GCN5 catalytic domain in 30 ul reaction system for 2 hours at room temperature. Products were detected by Western blot. H3ac (pan-acetyl) antibody (Cat. No. please inquire) was used to recognize acetylated histone H3.)
WB (Western Blot)
(Recombinant KAT2A (GCN5) protein activity assay0.5 ug Histone H3.1 (Cat. No. please inquire) was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) GCN5 catalytic domain in 30 ul reaction system for 2 hours at room temperature. Products were detected by Western blot. H3ac (pan-acetyl) antibody (Cat. No. please inquire) was used to recognize acetylated histone H3.)
SDS-PAGE
(Recombinant KAT2A (GCN5) protein, SDS-PAGE gel Recombinant KAT2A (GCN5) protein was run on a 10% SDS-PAGE gel and stained with Coomassie BlueMW: 95.6 kDa Purity: > 85%)
SDS-PAGE
(Recombinant KAT2A (GCN5) protein, SDS-PAGE gel Recombinant KAT2A (GCN5) protein was run on a 10% SDS-PAGE gel and stained with Coomassie BlueMW: 95.6 kDa Purity: > 85%)
Related Product Information for KAT2A recombinant protein
Background: GCN5 (General Control Of Amino Acid Synthesis Protein 5, also known as KAT2A, Lysine Acetyltransferase 2A) is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also acts as a repressor of NF-kappa-B by promoting ubiquitination of the NF-kappa-B subunit RELA in a HAT- independent manner. KAT2A (GCN5) has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. It also acetylates non-histone proteins, such as CEBPB. KAT2A (GCN5) is a core component of the ATAC complex, which is a complex with histone acetyltransferase activity on histones H3 and H4.
Product Categories/Family for KAT2A recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone acetyltransferase KAT2A
NCBI Official Synonym Full Names
K(lysine) acetyltransferase 2A
NCBI Official Symbol
KAT2A
NCBI Official Synonym Symbols
GCN5; hGCN5; GCN5L2; PCAF-b
NCBI Protein Information
histone acetyltransferase KAT2A; STAF97; hsGCN5; lysine acetyltransferase 2A; histone acetyltransferase GCN5; general control of amino acid synthesis protein 5-like 2; General control of amino acid synthesis, yeast, homolog-like 2; GCN5 (general control o
UniProt Protein Name
Histone acetyltransferase KAT2A
UniProt Gene Name
KAT2A
UniProt Synonym Gene Names
GCN5; GCN5L2; HGCN5; HsGCN5
UniProt Entry Name
KAT2A_HUMAN
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Product Notes
The KAT2A kat2a (Catalog #AAA59769) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KAT2A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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