Standard Curve (Sample)
Rat hepatitis A virus cellular receptor 1 ELISA Kit | Kim-1 elisa kit
Rat Kidney injury molecule 1, Kim-1 ELISA Kit
Gene Names
Havcr1; Kim1; KIM-1
Reactivity
Rat
Synonyms
hepatitis A virus cellular receptor 1; N/A; Rat Kidney injury molecule 1, Kim-1 ELISA Kit; Rat Kidney injury molecule 1; Kim-1 ELISA Kit; HAVCR; HAVCR-1; KIM-1; KIM1; TIM-1; TIM1; TIMD1; T cell immunoglobin domain and mucin domain protein 1; kidney injury molecule 1; Kim-1; Kim-1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat HAVCR1. No significant cross-reactivity or interference between Rat HAVCR1 and analogues was observed.
Sequence Length
307
Samples
Serum, plasma, urine
Detection Range
0.313 ng/ml-20 ng/ml
Sensitivity
0.228 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
others
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Kim-1 elisa kit
Introduction: Kim-1 (kidney injury molecule-1) is a type I transmembrane glycoprotein expressed on dedifferentiated renal proximal tubule epithelial cells undergoing regeneration after toxic or ischemic injury. The extracellular domain of Kim-1 is composed of an immunoglobulin-like domain topping a long mucin-like domain, a structure that points to a possible role in cell adhesion by homology to several known adhesion proteins. Two splice variants (a and b) of the human Kim-1 having identical extracellular domains, differ in their cytoplasmic domains and tissue distributions. In normal kidneys, Kim-1 is undetectable, but this molecule is abundantly expressed in PTC after ischemic and nephrotoxic injury. In mice, Kim-1 is upregulated in polycystic kidney disease especially in regions of the kidney where there is fibrosis. In humans and in rodents, Kim-1 is induced after ischemic or toxic acute tubular necrosis and the cleaved Kim-1 ectodomain is quantified in the urine as a biomarker for acute kidney injury. The early and abundant tubular expression following different types of injury makes Kim-1 a specific marker of tubular cell injury that may be linked to recuperative or damaging mechanisms that direct the process of interstitial damage.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kim-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Kim-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Kim-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Kim-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kim-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Kim-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Kim-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Kim-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,964 Da
NCBI Official Full Name
hepatitis A virus cellular receptor 1 homolog
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 1
NCBI Official Symbol
Havcr1
NCBI Official Synonym Symbols
Kim1; KIM-1
NCBI Protein Information
hepatitis A virus cellular receptor 1 homolog; TIMD-1; HAVcr-1; kidney injury molecule 1; t cell immunoglobulin and mucin domain-containing protein 1
UniProt Protein Name
Hepatitis A virus cellular receptor 1 homolog
UniProt Gene Name
Havcr1
UniProt Synonym Gene Names
Kim1; HAVcr-1; KIM-1; TIMD-1
UniProt Entry Name
HAVR1_RAT
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Product Notes
The Rat Kim-1 havcr1 (Catalog #AAA79696) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79696 ELISA Kit recognizes Rat Kim-1. It is sometimes possible for the material contained within the vial of "hepatitis A virus cellular receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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