SDS-PAGE
SCF recombinant protein
mouse SCF, 26-189aa, mouse, E Coli
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
SDS-PAGE
NCBI and Uniprot Product Information
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Product Notes
The SCF kitlg (Catalog #AAA11748) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SCF can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SCF kitlg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA. It is sometimes possible for the material contained within the vial of "SCF, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is SCF recombinant protein commonly used for in cell culture?
SCF (Stem Cell Factor), also known as c-kit ligand, is a growth factor widely used to support survival, proliferation, and differentiation of hematopoietic stem cells, progenitor cells, mast cells, and germ cells. It is also employed in stem cell culture systems (ESCs, iPSCs) to promote differentiation into specific lineages such as megakaryocytes, T cells, and natural killer cells.
Which cell types respond best to SCF stimulation?
Cell types with strong SCF responsiveness include TF-1 human erythroleukemic cells, LAD2 human mast cells, CD34+ hematopoietic stem/progenitor cells, and KIT-expressing myeloid cells. SCF acts via the KIT receptor tyrosine kinase, so cells expressing high KIT levels show optimal proliferative and survival responses to SCF stimulation.
What working concentration of SCF is typically used in assays?
Working concentrations of SCF in cell culture assays typically range from 10–100 ng/mL, though optimal concentrations vary by cell type and application. For hematopoietic stem cell culture, concentrations of 50–100 ng/mL are commonly used, while lower concentrations (10–50 ng/mL) may be sufficient for some progenitor cell populations.
How should SCF recombinant protein be reconstituted for best activity?
SCF should be reconstituted in sterile, pyrogen-free water or PBS containing protease inhibitors or stabilizing proteins (e.g., l-arginine, BSA). Gentle resuspension (avoiding vigorous vortexing) is recommended to minimize aggregation and loss of bioactivity. SCF contains disulfide bonds, so reconstitution buffers should not contain reducing agents unless the protein has been specially prepared.
Can SCF be used in serum-free or stem cell culture systems?
Yes, SCF is compatible with serum-free and defined culture systems, making it ideal for stem cell expansion and differentiation protocols. It is particularly valuable in animal-derived component-free (ADCF) media, where it supports long-term expansion of hematopoietic stem cells without requiring serum supplementation.
How should reconstituted SCF protein be stored to avoid loss of activity?
Reconstituted SCF should be stored at 2–8°C for short-term use (up to 2 weeks) or at -20°C to -80°C for long-term storage in small aliquots. Avoid repeated freeze-thaw cycles, which damage the protein structure. Adding BSA (0.1%) or similar protectants can improve stability and prevent aggregation during storage.
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