Application Data
Protein Transport Inhibitor MIX Kit
Protein Transport Inhibitor MIX
Synonyms
Protein Transport Inhibitor MIX; N/A; Protein Transport Inhibitor MIX kit
Reagent Preparation
1000×Protein Transport Inhibitor MIX: Add 50 uL absolute ethanol (self-prepared) to a vial of Protein Transport Inhibitor MIX and mix fully.
Note: Centrifuge at 2000~10000×g for several seconds before use and then open the cover for use. Absolute ethanol is volatile, please keep it sealed properly.
Note: Centrifuge at 2000~10000×g for several seconds before use and then open the cover for use. Absolute ethanol is volatile, please keep it sealed properly.
Experimental Procedure
1. Prepare the single cell suspension with complete medium (self-prepared), and adjust the cell density to 1~2×106/mL.
Note: The cell density should not be too high, and the maximum density should be less than 2×106/mL, high cell density will affect cell activation efficiency. Make sure the cells are in good condition before stimulation, especially for freshly prepared primary cells.
2. Add 2 uL of 500× Cell Stimulation MIX to each 1mL of cell suspension, and incubate the cells at 37 degree C, 5%CO2 for 1.5~1 h.
3. Add 1 uL of 1000×Protein Transport Inhibitor MIX to each 1mL of cell suspension, and incubate the cells at 37 degree C, 5%CO2 for 5~16 h (It is recommended to determine the optimal induction time by setting up a pre-experiment with different induction times for the cytokines to be tested. The common induction time can be refer to table 1).
4. Collect cell suspension, centrifuge at 200~300×g for 5 min, discard the supernatant and collect the cell pellet which could be used for subsequent intracellular factor detection after fixation.
Note: The cell density should not be too high, and the maximum density should be less than 2×106/mL, high cell density will affect cell activation efficiency. Make sure the cells are in good condition before stimulation, especially for freshly prepared primary cells.
2. Add 2 uL of 500× Cell Stimulation MIX to each 1mL of cell suspension, and incubate the cells at 37 degree C, 5%CO2 for 1.5~1 h.
3. Add 1 uL of 1000×Protein Transport Inhibitor MIX to each 1mL of cell suspension, and incubate the cells at 37 degree C, 5%CO2 for 5~16 h (It is recommended to determine the optimal induction time by setting up a pre-experiment with different induction times for the cytokines to be tested. The common induction time can be refer to table 1).
4. Collect cell suspension, centrifuge at 200~300×g for 5 min, discard the supernatant and collect the cell pellet which could be used for subsequent intracellular factor detection after fixation.
Preparation and Storage
Store at -20 degree C, shading light
Estimated Expiration: 12 months
Shipping: Ice bag
Estimated Expiration: 12 months
Shipping: Ice bag
Application Data
Application Data
Application Data
Application Data
Related Product Information for Protein Transport Inhibitor MIX kit
Background: Protein Transport Inhibitor MIX is mainly composed of Monensin and Brefeldin A, which can be used in combination with Cell Stimulation MIX to prevent the loss of cytokine transport. After cell membrane rupture, cytokines can be detected. It can also be used alone to block protein transport from the Golgi apparatus and endoplasmic reticulum in cells.
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Product Notes
The Protein Transport Inhibitor MIX (Catalog #AAA178126) is a Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Protein Transport Inhibitor MIX, Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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