Standard Curve (Sample)
Human Krueppel-like factor 4 ELISA Kit | KLF4 elisa kit
Human Krueppel-like factor 4 ELISA Kit
Gene Names
KLF4; EZF; GKLF
Reactivity
Human
Synonyms
Krueppel-like factor 4; N/A; Human Krueppel-like factor 4 ELISA Kit; KLF4 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of KLF4. No significant cross-reactivity or interference between KLF4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KLF4 and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
513
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for KLF4 elisa kit
Intended Uses: This KLF4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KLF4. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: KLF4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-KLF4 antibody and an KLF4-HRP conjugate. The assay sample and buffer are incubated together with KLF4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLF4 concentration since KLF4 from samples and KLF4-HRP conjugate compete for the anti-KLF4 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLF4 from the sample, fewer sites are left to bind KLF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLF4 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: KLF4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-KLF4 antibody and an KLF4-HRP conjugate. The assay sample and buffer are incubated together with KLF4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLF4 concentration since KLF4 from samples and KLF4-HRP conjugate compete for the anti-KLF4 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLF4 from the sample, fewer sites are left to bind KLF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLF4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
6,489 Da
NCBI Official Full Name
Krueppel-like factor 4 isoform 1
NCBI Official Synonym Full Names
Kruppel like factor 4
NCBI Official Symbol
KLF4
NCBI Official Synonym Symbols
EZF; GKLF
NCBI Protein Information
Krueppel-like factor 4
UniProt Protein Name
Krueppel-like factor 4
UniProt Gene Name
KLF4
UniProt Synonym Gene Names
EZF; GKLF
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Product Notes
The Human KLF4 klf4 (Catalog #AAA247186) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA247186 ELISA Kit recognizes Human KLF4. It is sometimes possible for the material contained within the vial of "Krueppel-like factor 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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