SDS-PAGE
(Recombinant KMT2A (MLL1)-SET binding assay. KMT2A (MLL1)-SET was incubated with 4 equimolar components of MLL1 complex (ASH1L, RBBP5, WDR5 and DPY30) for 2 hours at 4 degree C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.)
KMT2A recombinant protein
Recombinant KMT2A (MLL1)-SET protein
Gene Names
KMT2A; HRX; MLL; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL/GAS7; TET1-MLL
Purity
The recombinant protein is >92% pure by SDS-PAGE.
Synonyms
KMT2A; N/A; Recombinant KMT2A (MLL1)-SET protein; HRX; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL-AF9; MLL/GAS7; TET1-MLL; KMT2A recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >92% pure by SDS-PAGE.
Form/Format
20ug of Recombinant KMT2A (MLL1)-SET expressed in E Coli and supplied in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol, 0.04% Triton X-100.
Protein Species
Human
Tag
GST-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-PAGE
(Recombinant KMT2A (MLL1)-SET binding assay. KMT2A (MLL1)-SET was incubated with 4 equimolar components of MLL1 complex (ASH1L, RBBP5, WDR5 and DPY30) for 2 hours at 4 degree C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE
(Recombinant KMT2A (MLL1)-SET binding assay. KMT2A (MLL1)-SET was incubated with 4 equimolar components of MLL1 complex (ASH1L, RBBP5, WDR5 and DPY30) for 2 hours at 4 degree C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE
(Recombinant KMT2A (MLL1)-SET protein gel. KMT2A (MLL1)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE
(Recombinant KMT2A (MLL1)-SET protein gel. KMT2A (MLL1)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
Related Product Information for KMT2A recombinant protein
Short Description: The peptide corresponding to amino acids 3735-3973 of the KMT2A (MLL1) protein (accession number NP_001184033.1) was expressed in E Coli. The peptide contains the SET domain of MLL that is responsible for methyltransferase activity. Recombinant Recombinant KMT2A (MLL1)-SET contains an N-terminal GST-Tag with a molecular weight of 53.7 kDa. Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), or MLL, also known as HRX, is a Trithorax-group protein that function collectively to promote gene expression. MLL is a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The protein contains multiple conserved functional domains. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants. Recombinant KMT2A (MLL1)-SET protein can be used for enzyme kinetics and inhibitor screening studies.
Background: Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), or MLL, also known as KMT2A or HRX, is a Trithorax-group protein that function collectively to promote gene expression. MLL is a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The protein contains multiple conserved functional domains. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.
Background: Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), or MLL, also known as KMT2A or HRX, is a Trithorax-group protein that function collectively to promote gene expression. MLL is a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The protein contains multiple conserved functional domains. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.
Product Categories/Family for KMT2A recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
431,764 Da
NCBI Official Full Name
histone-lysine N-methyltransferase 2A isoform 1
NCBI Official Synonym Full Names
lysine (K)-specific methyltransferase 2A
NCBI Official Symbol
KMT2A
NCBI Official Synonym Symbols
HRX; MLL; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL/GAS7; TET1-MLL
NCBI Protein Information
histone-lysine N-methyltransferase 2A; trithorax-like protein; CDK6/MLL fusion protein; MLL/GAS7 fusion protein; MLL/GMPS fusion protein; zinc finger protein HRX; MLL-AF4 der(11) fusion protein; CXXC-type zinc finger protein 7; myeloid/lymphoid or mixed-l
UniProt Protein Name
Histone-lysine N-methyltransferase 2A
UniProt Gene Name
KMT2A
UniProt Synonym Gene Names
ALL1; CXXC7; HRX; HTRX; MLL; MLL1; TRX1; Lysine N-methyltransferase 2A; p320; p180
UniProt Entry Name
KMT2A_HUMAN
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Product Notes
The KMT2A kmt2a (Catalog #AAA59670) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KMT2A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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