Application Data
(Recombinant KMT2A (MLL1) complex activity assay. 3.3 ?M H3K4me0 (1-21aa) peptide was incubated with MLL1 complex in reaction buffer for 3 hour at room temperature. MLL1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
KMT2A recombinant protein
Recombinant KMT2A (MLL1) complex
Gene Names
KMT2A; HRX; MLL; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL/GAS7; TET1-MLL
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Synonyms
KMT2A; N/A; Recombinant KMT2A (MLL1) complex; KMT2A recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >95% pure by SDS-PAGE.
Form/Format
Full length Recombinant KMT2A (MLL1) complex is supplied in 25mM Tris pH8.0, 300mM NaCl, 5% Glycerol and 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.
Protein Species
Human
Tag
GST-Tag, His-Tag
Notes
Recombinant KMT2A (MLL1) complex is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4 methyltransferase Catalytic Ability: ~300 turnovers/ enzyme molecule HMT Assay Conditions: 3.3uM H3K4me0 (1-21aa) peptide was incubated with different concentration of Recombinant KMT2A (MLL1) complex in reaction buffer containing 50mM TrisCl pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 100uM SAM for 3 hours at room temperature. Activity was detected by HTRF and MALDI-TOF.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant KMT2A (MLL1) complex activity assay. 3.3 ?M H3K4me0 (1-21aa) peptide was incubated with MLL1 complex in reaction buffer for 3 hour at room temperature. MLL1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
Application Data
(Recombinant KMT2A (MLL1) complex activity assay. 3.3 ?M H3K4me0 (1-21aa) peptide was incubated with MLL1 complex in reaction buffer for 3 hour at room temperature. MLL1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
Application Data
(Recombinant KMT2A (MLL1) complex activity assay. 3.3 uM H3K4me0 peptide was incubated with 10 nM MLL1 complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control.)
Application Data
(Recombinant KMT2A (MLL1) complex activity assay. 3.3 uM H3K4me0 peptide was incubated with 10 nM MLL1 complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control.)
SDS-PAGE
(Recombinant KMT2A (MLL1) complex gel. Recombinant MLL1 complex was run on an 12% SDS-PAGE gel and stained with Coomassie blue.)
SDS-PAGE
(Recombinant KMT2A (MLL1) complex gel. Recombinant MLL1 complex was run on an 12% SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for KMT2A recombinant protein
Short Description: Recombinant KMT2A (MLL1) complex contains amino acids 3735-3973 of the human MLL1 protein (accession number NP_001184033.1) with N-terminal GST-Tag and MW = 53.7 kDa; full-length human WDR5 (accession number NM_017588), with N-terminal 6xHis-tag and MW = 35 kDa; full-length human ASH2L (accession number NM_001105214), N-terminal 6xHis-tag and MW = 61 kDa; full-length human RbBP5 (accession number NM_005057), N-terminal 6xHis-tag and MW = 60 kDa, and full-length human DPY30 (accession number NM_032574), N-terminal 6xHis-tag and MW =12 kDa, all individually expressed in E Coli. The recombinant protein is >=95% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: Recombinant KMT2A (MLL1) complex (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog 1, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1 /MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis.
Background: Recombinant KMT2A (MLL1) complex (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog 1, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1 /MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis.
Product Categories/Family for KMT2A recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
431,764 Da
NCBI Official Full Name
histone-lysine N-methyltransferase 2A isoform 1
NCBI Official Synonym Full Names
lysine (K)-specific methyltransferase 2A
NCBI Official Symbol
KMT2A
NCBI Official Synonym Symbols
HRX; MLL; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL/GAS7; TET1-MLL
NCBI Protein Information
histone-lysine N-methyltransferase 2A; trithorax-like protein; CDK6/MLL fusion protein; MLL/GAS7 fusion protein; MLL/GMPS fusion protein; zinc finger protein HRX; MLL-AF4 der(11) fusion protein; CXXC-type zinc finger protein 7; myeloid/lymphoid or mixed-l
UniProt Protein Name
Histone-lysine N-methyltransferase 2A
UniProt Gene Name
KMT2A
UniProt Synonym Gene Names
ALL1; CXXC7; HRX; HTRX; MLL; MLL1; TRX1; Lysine N-methyltransferase 2A; p320; p180
UniProt Entry Name
KMT2A_HUMAN
Similar Products
Product Notes
The KMT2A kmt2a (Catalog #AAA59674) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KMT2A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.