Application Data
(Recombinant KMT2C (MLL3) complex activity assay. 3.3 uM H3K4me0 peptide was incubated with 10 nM Recombinant KMT2C (MLL3) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~165 turnovers/ enzyme molecule)
KMT2C recombinant protein
Recombinant KMT2C (MLL3) complex
Gene Names
KMT2C; HALR; MLL3
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Synonyms
KMT2C; N/A; Recombinant KMT2C (MLL3) complex; HALR; KMT2C recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >90% pure by SDS-PAGE.
Form/Format
Full length Recombinant KMT2C (MLL3) complex expressed in E Coli cells and supplied in 25mM Tris pH8.0, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.
Protein Species
Human
Tag
GST-Tag, His-Tag
Notes
Recombinant KMT2C (MLL3) complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions:50mM TrisCl, pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 100uM SAM, 10nm Recombinant KMT2C (MLL3) complex, 3.3uM H3 (1-21) peptide at 2 hours at room temperature.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant KMT2C (MLL3) complex activity assay. 3.3 uM H3K4me0 peptide was incubated with 10 nM Recombinant KMT2C (MLL3) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~165 turnovers/ enzyme molecule)
Application Data
(Recombinant KMT2C (MLL3) complex activity assay. 3.3 uM H3K4me0 peptide was incubated with 10 nM Recombinant KMT2C (MLL3) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~165 turnovers/ enzyme molecule)
Application Data
(Recombinant KMT2C (MLL3) complex activity assay. 3.3 uM H3K4me0 (1-21aa) peptide was incubated with Recombinant KMT2C (MLL3) complex in reaction buffer for 3 hour at room temperature. Recombinant KMT2C (MLL3) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
Application Data
(Recombinant KMT2C (MLL3) complex activity assay. 3.3 uM H3K4me0 (1-21aa) peptide was incubated with Recombinant KMT2C (MLL3) complex in reaction buffer for 3 hour at room temperature. Recombinant KMT2C (MLL3) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
SDS-PAGE
(Recombinant KMT2C (MLL3) complex protein gel. Recombinant KMT2C (MLL3) complex run on an SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE
(Recombinant KMT2C (MLL3) complex protein gel. Recombinant KMT2C (MLL3) complex run on an SDS-PAGE gel and stained with Coomassie Blue.)
Related Product Information for KMT2C recombinant protein
Short Description: Recombinant KMT2C (MLL3) complex (MLL3/WDR5/ASH2L/RBBP5/DPY30) was generated using a peptide corresponding to amino acids 4740-4911 (accession number NP_733751.2) of the KMT2C protein that was expressed in E Coli. Recombinant KMT2C (MLL3) complex contains an N-terminal GST-Tag with a molecular weight of 46 kDa; human WDR5 (accession number NM_017588) that contains an N-terminal His-Tag with a molecular weight of 35 kDa; human ASH2L (accession number NM_001105214) that contains an N-terminal His-Tag with a molecular weight of 61 kDa; human RBBP5 (accession number NM_005057) that contains an N-terminal His-Tag with a molecular weight of 60 kDa; and human DPY30 (accession number NM_032574) that contains an N-terminal His-Tag with a molecular weight of 12 kDa. All Recombinant MLL3/WDR5/ASH2L/RBBP5/DPY30 complex proteins were individually expressed in an E Coli expression system. The recombinant protein is >90% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: The KMT2C (MLL3) complex (MLL3/WDR5/ASH2L/RBBP5/DPY30), also known as the MLL3 WARD Complex is a histone methyltransferase that, like MLLs 1, 2 and 4, preferentially methylates the activating histone mark lysine 4 of histone H3 (H3K4). The KMT2C (also known as MLL3) subunit contains the catalytic SET domain that is responsible for substrate recognition and enzymatic activity, along with the WDR5 and RBBP5 interaction motifs necessary for assembly of the WARD complex. The MLL3 WARD complex forms part of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. The chromatin remodeling complex SWI/SNF also interacts with ASCOM through binding of the MLL3 (or MLL4) SET domain with the INI1 subunit of SWI/SNF. Knockdown of MLLs 3 and 4 expression results in a reduction of HOXC10, an estrogen-induced protein that is overexpressed in breast cancer.
Background: The KMT2C (MLL3) complex (MLL3/WDR5/ASH2L/RBBP5/DPY30), also known as the MLL3 WARD Complex is a histone methyltransferase that, like MLLs 1, 2 and 4, preferentially methylates the activating histone mark lysine 4 of histone H3 (H3K4). The KMT2C (also known as MLL3) subunit contains the catalytic SET domain that is responsible for substrate recognition and enzymatic activity, along with the WDR5 and RBBP5 interaction motifs necessary for assembly of the WARD complex. The MLL3 WARD complex forms part of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. The chromatin remodeling complex SWI/SNF also interacts with ASCOM through binding of the MLL3 (or MLL4) SET domain with the INI1 subunit of SWI/SNF. Knockdown of MLLs 3 and 4 expression results in a reduction of HOXC10, an estrogen-induced protein that is overexpressed in breast cancer.
Product Categories/Family for KMT2C recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
541,370 Da
NCBI Official Full Name
histone-lysine N-methyltransferase 2C
NCBI Official Synonym Full Names
lysine (K)-specific methyltransferase 2C
NCBI Official Symbol
KMT2C
NCBI Official Synonym Symbols
HALR; MLL3
NCBI Protein Information
histone-lysine N-methyltransferase 2C; ALR-like protein; homologous to ALR protein; histone-lysine N-methyltransferase MLL3; myeloid/lymphoid or mixed-lineage leukemia protein 3; histone-lysine N-methyltransferase, H3 lysine-4 specific
UniProt Protein Name
Histone-lysine N-methyltransferase 2C
UniProt Gene Name
KMT2C
UniProt Synonym Gene Names
HALR; KIAA1506; MLL3; Lysine N-methyltransferase 2C
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Product Notes
The KMT2C kmt2c (Catalog #AAA59708) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KMT2C, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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