Application Data
(Recombinant KMT2D (MLL2) complex MALDI-TOF activity assay 3.3 uM H3K4me0 peptide was incubated with 10 nM KMT2D (MLL2) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~450 turnovers/ enzyme molecule)
KMT2D recombinant protein
Recombinant KMT2D (MLL2) complex
Gene Names
KMT2D; ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
Purity
The recombinant protein is >80% pure by SDS-PAGE.
Synonyms
KMT2D; N/A; Recombinant KMT2D (MLL2) complex; KMT2D recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >80% pure by SDS-PAGE.
Form/Format
Recombinant KMT2D (MLL2) complex was expressed in E Coli cells and is supplied in 25mM Tris pH8.0, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100.
Protein Species
Human
Tag
GST-Tag, His-Tag
Notes
Recombinant KMT2D (MLL2) complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 3.3uM H3K4me0 (1-21aa) peptide was incubated with different concentrations of recombinant KMT2D (MLL2) complex in reaction buffer containing 50mM TrisCl pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 100uM SAM for 3 hours at room temperature. Activity was detected by HTRF and MALDI-TOF.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant KMT2D (MLL2) complex MALDI-TOF activity assay 3.3 uM H3K4me0 peptide was incubated with 10 nM KMT2D (MLL2) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~450 turnovers/ enzyme molecule)
Application Data
(Recombinant KMT2D (MLL2) complex MALDI-TOF activity assay 3.3 uM H3K4me0 peptide was incubated with 10 nM KMT2D (MLL2) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~450 turnovers/ enzyme molecule)
Application Data
(Recombinant KMT2D (MLL2) complex HTRF activity assay 3.3 uM H3K4me0 (1-21aa) peptide was incubated with KMT2D (MLL2) complex in reaction buffer for 3 hour at room temperature. KMT2D (MLL2) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
Application Data
(Recombinant KMT2D (MLL2) complex HTRF activity assay 3.3 uM H3K4me0 (1-21aa) peptide was incubated with KMT2D (MLL2) complex in reaction buffer for 3 hour at room temperature. KMT2D (MLL2) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
SDS-PAGE
(Recombinant KMT2D (MLL2) complex gel Recombinant KMT2D (MLL2) complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.)
SDS-PAGE
(Recombinant KMT2D (MLL2) complex gel Recombinant KMT2D (MLL2) complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for KMT2D recombinant protein
Short Description: Recombinant KMT2D (MLL2) complex contains amino acids 5355-5537 of the human MLL2 protein (accession number NP_003473.3) with N-terminal GST-Tag and MW = 47.6 kDa; full-length human WDR5 (accession number NM_017588), with N-terminal 6xHis-tag and MW = 35 kDa; full-length human ASH2L (accession number NM_001105214), N-terminal 6xHis-tag and MW = 61 kDa; full-length human RbBP5 (accession number NM_005057), N-terminal 6xHis-tag and MW = 60 kDa, and full-length human DPY30 (accession number NM_032574), N-terminal 6xHis-tag and MW =12 kDa, all individually expressed in E Coli. The recombinant protein is >80% pure by SDS-PAGE. Recombinant KMT2D (MLL2) complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: KMT2D (MLL2) complex (Myeloid/lymphoid or mixed-lineage leukemia 2) is a Trithorax-group protein that function collectively to promote gene expression. KMT2D (MLL2) is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL2 is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the b-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome.
Background: KMT2D (MLL2) complex (Myeloid/lymphoid or mixed-lineage leukemia 2) is a Trithorax-group protein that function collectively to promote gene expression. KMT2D (MLL2) is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL2 is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the b-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome.
Product Categories/Family for KMT2D recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
593,677 Da
NCBI Official Full Name
histone-lysine N-methyltransferase 2D
NCBI Official Synonym Full Names
lysine (K)-specific methyltransferase 2D
NCBI Official Symbol
KMT2D
NCBI Official Synonym Symbols
ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
NCBI Protein Information
histone-lysine N-methyltransferase 2D; ALL1-related protein; Kabuki make-up syndrome; Kabuki mental retardation syndrome; trinucleotide repeat containing 21; histone-lysine N-methyltransferase MLL2; myeloid/lymphoid or mixed-lineage leukemia 2
UniProt Protein Name
Histone-lysine N-methyltransferase 2D
UniProt Gene Name
KMT2D
UniProt Synonym Gene Names
ALR; MLL2; MLL4; Lysine N-methyltransferase 2D
UniProt Entry Name
KMT2D_HUMAN
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Product Notes
The KMT2D kmt2d (Catalog #AAA59719) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KMT2D, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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