Application Data
SUV420H1 recombinant protein
SUV420H1 (KMT5B), Active
Gene Names
KMT5B; CGI85; CGI-85; SUV420H1
Applications
Methyltransferase Assay
Synonyms
SUV420H1; N/A; SUV420H1 (KMT5B), Active; SUV420H1 recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
Full length
Sequence Length
2771
Applicable Applications for SUV420H1 recombinant protein
Methyltransferase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for SUV420H1 recombinant protein
Description: Recombinant full-length human SUV420H1 (KMT5B) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: Histone-lysine N-methyltransferase, Suppressor of variegation 4-20 homolog 1 SUV420H1 specifically trimethylates 'Lys-20' of histone H4, which in turn acts as a specific tag for epigenetic transcriptional repression. Located in pericentric heterochromatin regions, and plays a central role in the establishment of constitutive heterochromatin in these regions. Interacts with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases) mediated by its SET domains.
Scientific Background: Histone-lysine N-methyltransferase, Suppressor of variegation 4-20 homolog 1 SUV420H1 specifically trimethylates 'Lys-20' of histone H4, which in turn acts as a specific tag for epigenetic transcriptional repression. Located in pericentric heterochromatin regions, and plays a central role in the establishment of constitutive heterochromatin in these regions. Interacts with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases) mediated by its SET domains.
Product Categories/Family for SUV420H1 recombinant protein
References
1. Tryndyak VP, et al: Loss of DNA methylation and histone H4 lysine 20 trimethylation in human breast cancer cells is associated with aberrant expression of DNA methyl-transferase 1, Suv4-20h2 histone methyltransferase and methyl-binding proteins. Cancer Biol Ther. 5 (1):65-70, 2006. 2. Lai CH, et al: Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. Genome Res. 10 (5):703-13, 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
160 kDa
NCBI Official Full Name
Homo sapiens suppressor of variegation 4-20 homolog 1 (Drosophila), transcript variant 1, mRNA
NCBI Official Synonym Full Names
lysine methyltransferase 5B
NCBI Official Symbol
KMT5B
NCBI Official Synonym Symbols
CGI85; CGI-85; SUV420H1
NCBI Protein Information
histone-lysine N-methyltransferase KMT5B
UniProt Protein Name
Histone-lysine N-methyltransferase KMT5B
UniProt Gene Name
KMT5B
UniProt Synonym Gene Names
Su(var)4-20 homolog 1; Suv4-20h1
Similar Products
Product Notes
The SUV420H1 kmt5b (Catalog #AAA73136) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's SUV420H1 can be used in a range of immunoassay formats including, but not limited to, Methyltransferase Assay. Researchers should empirically determine the suitability of the SUV420H1 kmt5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUV420H1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.