SDS-PAGE
Lactose Operon Repressor/LacI Recombinant Protein | LacI recombinant protein
Recombinant E Coli Lactose Operon Repressor/LacI Protein (His Tag)
Purity
>95 % as determined by reducing SDS-PAGE.
Synonyms
Lactose Operon Repressor/LacI; N/A; Recombinant E Coli Lactose Operon Repressor/LacI Protein (His Tag); Lactose operon repressor; LacI; LacI recombinant protein
Host
E Coli
Purity/Purification
>95 % as determined by reducing SDS-PAGE.
Form/Format
Lyophilized from sterile PBS, pH7.5, 5%trehalose, 5% mannitol, 0.01% Tween80 and 0.01% Proclin300.
Sequence
Met1-Gln360
Species
E Coli
Tag
C-His
Endotoxin
<1.0 EU per ug of the protein as determined by the LAL method.
Reconstution
recommends centrifuging the vial before opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water be added to the vial to prepare a stock solution of 1mg/mL. Concentration is measured by UV-Vis.
Preparation and Storage
Lyophilized protein should be stored at <-20°C, Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at <-20C for 3 months.
SDS-PAGE
SDS-PAGE
Related Product Information for LacI recombinant protein
Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Mol_Mass 39.4 kDa
AP_Mol_Mass 40 kDa
AP_Mol_Mass 40 kDa
NCBI Official Full Name
lactose-inducible lac operon transcriptional repressor
NCBI Official Symbol
lacI
NCBI Protein Information
DNA-binding transcriptional repressor
UniProt Protein Name
Lactose operon repressor
UniProt Gene Name
lacI
UniProt Entry Name
LACI_ECOLI
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Product Notes
The LacI laci (Catalog #AAA22243) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Gln36 0. It is sometimes possible for the material contained within the vial of "Lactose Operon Repressor/LacI, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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