Standard Curve (Sample)
Human Transcriptional repressor CTCF ELISA Kit | CTCF elisa kit
Human Transcriptional repressor CTCF ELISA Kit
Gene Names
CTCF; MRD21
Reactivity
Human
Synonyms
Transcriptional repressor CTCF; N/A; Human Transcriptional repressor CTCF ELISA Kit; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog; CTCF; CTCF elisa kit
Reactivity
Human
Specificity
Recombinant and natural Human Transcriptional repressor CTCF
Sequence Length
399
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Assay Type
Quantitative Competitive
Detection Range
0.312-20 ng/mL
Sensitivity
< 0.172 ng/mL
Intra-assay Precision
<=6.1%
Inter-assay Precision
<=10.2%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CTCF elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,998 Da
NCBI Official Full Name
transcriptional repressor CTCF isoform 2
NCBI Official Synonym Full Names
CCCTC-binding factor (zinc finger protein)
NCBI Official Symbol
CTCF
NCBI Official Synonym Symbols
MRD21
NCBI Protein Information
transcriptional repressor CTCF
UniProt Protein Name
Transcriptional repressor CTCF
UniProt Gene Name
CTCF
UniProt Entry Name
CTCF_HUMAN
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Product Notes
The Human CTCF ctcf (Catalog #AAA192570) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA192570 ELISA Kit recognizes Human CTCF. It is sometimes possible for the material contained within the vial of "Transcriptional repressor CTCF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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