Human lecithin-cholesterol acyltransferase ELISA Kit | LCAT elisa kit
Human Lecithin Cholesterol Acyltransferase, LCat ELISA Kit
Reactivity
Human
Synonyms
lecithin-cholesterol acyltransferase; N/A; Human Lecithin Cholesterol Acyltransferase, LCat ELISA Kit; Human Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit; LCAT elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human LCAT. No significant cross-reactivity or interference between human LCAT and analogues was observed.
Sequence Length
440
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
3.12 ng/ml-200 ng/ml
Sensitivity
< 0.78 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for LCAT elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LCAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LCAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,578 Da
NCBI Official Full Name
phosphatidylcholine-sterol acyltransferase
NCBI Official Synonym Full Names
lecithin-cholesterol acyltransferase
NCBI Official Symbol
LCAT
NCBI Protein Information
phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase
UniProt Protein Name
Phosphatidylcholine-sterol acyltransferase
UniProt Gene Name
LCAT
