Human Leptin CLIA Kit | LEP clia kit

Leptin (Human) LumiAb ELISA Kit

Cat. Num. AAA318549

• Gene Names: LEP; OB; OBS; LEPD
• Reactivity: Human
• Synonyms: Leptin; N/A; Leptin (Human) LumiAb ELISA Kit; Human Leptin; Obese protein; Obesity factor; OB; OBS; LEP; LEP clia kit

• Reactivity: Human
• Specificity: The LumiAb Human I-TAC ELISA is capable of recognizing both recombinant and naturally produced Human I-TAC proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Sequence Length: 167
• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for LEP clia kit

Principle of the Assay: The LumiAb Human I-TAC Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human I-TAC concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human I-TAC while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human I-TAC or Interferon-Inducible T-Cell Alpha Chemoattractant, also known as C-X-C Motif Chemokine 11, is a 94 amino acid cytokine protein that displays chemotactic activity for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. I-TAC may also induce calcium release in activated T-cells via binding to CXCR3 receptor and also may play an important role in CNS diseases which involve T-cell recruitment as well as skin immune responses. There is typically high expression of I-TAC in peripheral blood leukocytes, pancreas and liver astrocytes while moderate levels of the chemokine are found in the thymus, lung and spleen. Low levels of I-TAC are typically found in placenta, the prostate and small intestine. Induction is usually facilitated by IFN-gamma and IFN-beta where induction by IFN-gamma is enhanced by TNF in monocytes, dermal fibroblasts and endothelial cells, and by IL-1 in astrocytes. In addition, the I-TAC chemokine is encoded by the CXCL11 gene located at locus 4q21.2 on chromosome 4.

Product Categories/Family for LEP clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 4557715
• NCBI GeneID: 3952
• NCBI Accession #: NP_000221.1
• NCBI GenBank Nucleotide #: NM_000230.3
• UniProt Accession #: P41159
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: LEP
• NCBI Official Synonym Symbols: OB; OBS; LEPD
• NCBI Protein Information: leptin
• UniProt Protein Name: Leptin
• UniProt Gene Name: LEP
• UniProt Synonym Gene Names: OB; OBS
• UniProt Entry Name: LEP_HUMAN