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product-image-AAA126772_SC15.png Standard Curve (Sample) (Human Leptin EZ Set ELISA Kit standard curve)

Human Leptin DIY ELISA Kit | LEP diy elisa kit

Human Leptin ELISA Kit EZ-Set (DIY Antibody Pairs)

Reactivity
Human
Applications
ELISA
Synonyms
Leptin; N/A; Human Leptin ELISA Kit EZ-Set (DIY Antibody Pairs); Ngfr; Tnfrsf16; Tumor necrosis factor receptor superfamily member 16; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; NGF receptor; CD antigen CD271; LEP diy elisa kit
Ordering
Reactivity
Human
Samples
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Detection Range
62.5pg/ml-4,000pg/ml
Sensitivity
<10pg/ml
Anticoagulant
heparin or EDTA
Subcellular Localization
Secreted.
Applicable Applications for LEP diy elisa kit
ELISA
Immunogen
Expression system for standard: E Coli; Immunogen sequence: V22-C167
Protein Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Human Leptin EZ Set ELISA Kit standard curve)

product-image-AAA126772_SC15.png Standard Curve (Sample) (Human Leptin EZ Set ELISA Kit standard curve)
Related Product Information for LEP diy elisa kit
Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.
Product Categories/Family for LEP diy elisa kit

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
18641MW
UniProt Protein Name
Leptin
UniProt Gene Name
LEP
UniProt Entry Name
A4D0Y8_HUMAN

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Product Notes

The Human LEP lep (Catalog #AAA126772) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126772 DIY ELISA Kit recognizes Human LEP. AAA Biotech's Leptin can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the LEP lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leptin, DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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