Monkey Leptin ELISA Kit | LEP elisa kit
Monkey Leptin (LEP) ELISA Kit
Gene Names
LEP; OB
Reactivity
Monkey
Synonyms
Leptin; N/A; Monkey Leptin (LEP) ELISA Kit; FLJ94114; OB; OBS; leptin (murine obesity homolog)|leptin (obesity homolog; mouse)|obese; mouse; homolog of|obesity factor; LEP elisa kit
Reactivity
Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of Monkey LEP. No significant cross-reactivity or interference between Monkey LEP and analogues was observed.
Sequence Length
167
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
2.5 ng/mL - 40 ng/mL
Sensitivity
2.5 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for LEP elisa kit
Intended Uses: For the quantitative detection of Monkey Leptin (LEP) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEP. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated LEP. A competitive inhibition reaction is launched between LEP (Standards or samples) and biotin-conjugated LEP with the pre-coated antibody specific for LEP. The more amount of LEP in samples, the less antibody bound by biotin-conjugated LEP. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of LEP in the sample. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEP. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated LEP. A competitive inhibition reaction is launched between LEP (Standards or samples) and biotin-conjugated LEP with the pre-coated antibody specific for LEP. The more amount of LEP in samples, the less antibody bound by biotin-conjugated LEP. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of LEP in the sample. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,953 Da
NCBI Official Full Name
leptin
NCBI Official Symbol
LEP
NCBI Official Synonym Symbols
OB
NCBI Protein Information
leptin
UniProt Protein Name
Leptin
UniProt Gene Name
LEP
UniProt Synonym Gene Names
OB
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Product Notes
The Monkey LEP lep (Catalog #AAA55828) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA55828 ELISA Kit recognizes Monkey LEP. It is sometimes possible for the material contained within the vial of "Leptin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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