Human Galectin-3 CLIA Kit | LGALS3 clia kit

Galectin-3 (Human) LumiAb ELISA Kit

Cat. Num. AAA318605

• Gene Names: LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• Reactivity: Human
• Synonyms: Galectin-3; N/A; Galectin-3 (Human) LumiAb ELISA Kit; Human Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Fractalkine ELISA is capable of recognizing both recombinant and naturally produced Human Fractalkine proteins. The antigens listed below were tested at 500 ng/ml and did not exhibit significant cross-reactivity or interference.
• Sequence Length: 250
• Assay Type: Quantitative Sandwich
• Sensitivity: 80 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for LGALS3 clia kit

Principle of the Assay: The LumiAb Human Fractalkine Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Fractalkine concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Fractalkine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Fractalkine, also known as C-X3-C motif chemokine 1 (CX3CL1), is a 397 amino acid single-pass type I membrane protein. The processed fractalkine is secreted. Fractalkine belongs to the intercrine delta family. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. Fractalkine may play a role in regulating leukocyte adhesion and migration processes at the endothelium. It binds to CX3CR1 and can be found in the small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Fractalkine is induced by TNF and IL1/interleukin-1 in pulmonary endothelial cells and umbilical vein endothelial cells. A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form. Fractalkine is O-glycosylated with core 1 or possibly core 8 glycans after post-translational modification.

Product Categories/Family for LGALS3 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 115430223
• NCBI GeneID: 3958
• NCBI Accession #: NP_002297.2
• NCBI GenBank Nucleotide #: NM_002306.4
• UniProt Accession #: P17931
• NCBI Official Full Name: galectin-3 isoform 1
• NCBI Official Synonym Full Names: galectin 3
• NCBI Official Symbol: LGALS3
• NCBI Official Synonym Symbols: L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• NCBI Protein Information: galectin-3
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: LGALS3
• UniProt Synonym Gene Names: MAC2; Gal-3; CBP 35; GALBP
• UniProt Entry Name: LEG3_HUMAN