Human Galectin-3/LGALS3 ELISA Kit | LGALS3 elisa kit

Human Galectin-3/LGALS3 PicoKine ELISA Kit

Cat. Num. AAA45529

• Gene Names: LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• Reactivity: Human
• Synonyms: Galectin-3/LGALS3; N/A; Human Galectin-3/LGALS3 PicoKine ELISA Kit; Galectin-3; lectin, galactoside-binding, soluble, 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; GALIG; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; LGALS2Mac-2 antigen; MAC2; Macrophage galactose-specific lectin; LGALS3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Galectin-3
• Sequence Length: 250
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml

• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human Galectin-3/LGALS3 PicoKine ELISA Kit standard curve)

Related Product Information for LGALS3 elisa kit

Principle of the assay: human Galectin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-3 has been precoated onto 96-well plates. Standards(E.coli,A2-I250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-3 amount of sample captured in plate.
Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 215274262
• NCBI GeneID: 3958
• UniProt Accession #: P17931; Q16005; Q6IBA7; Q96J47; B2RC38
• Molecular Weight: 26,152 Da
• NCBI Official Full Name: Galectin-3
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 3
• NCBI Official Symbol: LGALS3
• NCBI Official Synonym Symbols: L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• NCBI Protein Information: galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: LGALS3
• UniProt Synonym Gene Names: MAC2; Gal-3; CBP 35; GALBP
• UniProt Entry Name: LEG3_HUMAN