Application Data
LIMK1 recombinant protein
LIMK1, Active
Gene Names
LIMK1; LIMK; LIMK-1
Applications
Western Blot
Synonyms
LIMK1; N/A; LIMK1, Active; LIMK1 recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
285-638
Sequence Length
3398
Applicable Applications for LIMK1 recombinant protein
WB (Western Blot), Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for LIMK1 recombinant protein
Description: Recombinant human LIMK1 (285-638) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.
Scientific Background: LIMK1 or LIM domain kinase 1 contains a unique combination of 2 N-terminal LIM domain and a C-terminal protein kinase domain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers that can bind to DNA/RNA as well as mediating protein-protein interactions (1). LIMK1 is thought to be a component of an intracellular signaling pathway that may be involved in brain development especially development of nerve cells. LIMK1 may play an important role in areas of the brain that are responsible for processing visual-spatial information (visuospatial constructive cognition). LIMK1 can regulate aspects of the cytoskeleton, the structural framework that helps to determine cell shape, size, and movement (2).
Scientific Background: LIMK1 or LIM domain kinase 1 contains a unique combination of 2 N-terminal LIM domain and a C-terminal protein kinase domain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers that can bind to DNA/RNA as well as mediating protein-protein interactions (1). LIMK1 is thought to be a component of an intracellular signaling pathway that may be involved in brain development especially development of nerve cells. LIMK1 may play an important role in areas of the brain that are responsible for processing visual-spatial information (visuospatial constructive cognition). LIMK1 can regulate aspects of the cytoskeleton, the structural framework that helps to determine cell shape, size, and movement (2).
Product Categories/Family for LIMK1 recombinant protein
References
1. Davila, M. et al: LIM kinase 1 is essential for the invasive growth of prostate epithelial cells: implications in prostate cancer. J Biol Chem. 2003;19;278 (38):36868-75. 2. Davila, M. et al: Expression of LIM kinase 1 is associated with reversible G1/S phase arrest, chromosomal instability and prostate cancer. Mol Cancer. 2007;8;6:40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~65 kDa
NCBI Official Full Name
Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
LIM domain kinase 1
NCBI Official Symbol
LIMK1
NCBI Official Synonym Symbols
LIMK; LIMK-1
NCBI Protein Information
LIM domain kinase 1
UniProt Protein Name
LIM domain kinase 1
UniProt Gene Name
LIMK1
UniProt Synonym Gene Names
LIMK; LIMK-1
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Product Notes
The LIMK1 limk1 (Catalog #AAA73003) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 285-638. AAA Biotech's LIMK1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the LIMK1 limk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIMK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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