Standard Curve (Sample)
Human Laminin ELISA Kit | LN elisa kit
Human Laminin ELISA Kit
Gene Names
LAMC2; B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
Reactivity
Human
Synonyms
Laminin; N/A; Human Laminin ELISA Kit; LN elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Highest Standards
5000pg/mL
Concentration of Standards
Concentration of Standards
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LN elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This LN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LN. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY LN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LN. Standards or samples are then added to the microtiter plate wells and LN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LN are added to each well to “sandwich” the LN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LN concentration in each sample is interpolated from this standard curve.
INTENDED USE This LN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LN. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY LN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LN. Standards or samples are then added to the microtiter plate wells and LN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LN are added to each well to “sandwich” the LN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LN concentration in each sample is interpolated from this standard curve.
Product Categories/Family for LN elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
121,604 Da
NCBI Official Full Name
Laminin
NCBI Official Synonym Full Names
laminin, gamma 2
NCBI Official Symbol
LAMC2
NCBI Official Synonym Symbols
B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
NCBI Protein Information
laminin subunit gamma-2; BM600-100kDa; laminin B2t chain; CSF 140 kDa subunit; nicein subunit gamma; kalinin subunit gamma; ladsin 140 kDa subunit; epiligrin subunit gamma; cell-scattering factor 140 kDa subunit; large adhesive scatter factor 140 kDa subunit
UniProt Protein Name
Laminin subunit gamma-2
UniProt Gene Name
LAMC2
UniProt Synonym Gene Names
LAMB2T; LAMNB2; CSF 140 kDa subunit
UniProt Entry Name
LAMC2_HUMAN
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Product Notes
The Human LN lamc2 (Catalog #AAA84820) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA84820 ELISA Kit recognizes Human LN. It is sometimes possible for the material contained within the vial of "Laminin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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