Mouse Leucyl-Cystinyl aminopeptidase ELISA Kit | LNPEP elisa kit

Mouse Leucyl-Cystinyl aminopeptidase ELISA Kit

Cat. Num. AAA95772

• Gene Names: LNPEP; CAP; IRAP; PLAP; P-LAP
• Reactivity: Mouse
• Synonyms: Leucyl-Cystinyl aminopeptidase; N/A; Mouse Leucyl-Cystinyl aminopeptidase ELISA Kit; LNPEP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of LNPEP. No significant cross-reactivity or interference between LNPEP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LNPEP and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for LNPEP elisa kit

Principle of the Assay: LNPEP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LNPEP antibody and an LNPEP-HRP conjugate. The assay sample and buffer are incubated together with LNPEP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LNPEP concentration since LNPEP from samples and LNPEP-HRP conjugate compete for the anti-LNPEP antibody binding site. Since the number of sites is limited, as more sites are occupied by LNPEP from the sample, fewer sites are left to bind LNPEP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LNPEP concentration in each sample is interpolated from this standard curve.

Intended Uses: This LNPEP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse LNPEP. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 61742777
• NCBI GeneID: 4012
• NCBI Accession #: NP_005566.2
• NCBI GenBank Nucleotide #: NM_005575.2
• Molecular Weight: 115,062 Da
• NCBI Official Full Name: leucyl-cystinyl aminopeptidase isoform 1
• NCBI Official Synonym Full Names: leucyl/cystinyl aminopeptidase
• NCBI Official Symbol: LNPEP
• NCBI Official Synonym Symbols: CAP; IRAP; PLAP; P-LAP
• NCBI Protein Information: leucyl-cystinyl aminopeptidase; otase; oxytocinase; vasopressinase; AT (4) receptor; angiotensin IV receptor; insulin-regulated aminopeptidase; placental leucine aminopeptidase; insulin-responsive aminopeptidase; insulin-regulated membrane aminopeptidase
• UniProt Protein Name: Leucyl-cystinyl aminopeptidase
• UniProt Gene Name: LNPEP
• UniProt Synonym Gene Names: OTASE; Cystinyl aminopeptidase; IRAP; OTase; P-LAP
• UniProt Entry Name: LCAP_HUMAN