Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2?g/mL can bind Anti-ALPG recombinant antibody, the EC50 is 14.09-23.17 ng/mL.)
Alkaline phosphatase, germ cell type (ALPG) Active Protein | ALPG active protein
Recombinant Human Alkaline phosphatase, germ cell type (ALPG)(Active)
Gene Names
ALPPL2; ALPG; GCAP; ALPPL
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
Alkaline phosphatase, germ cell type (ALPG); N/A; Recombinant Human Alkaline phosphatase, germ cell type (ALPG)(Active); ALP-1; Alkaline phosphatase Nagao isozyme; Alkaline phosphatase, placental-like; Germ cell alkaline phosphatase; Placental alkaline phosphatase-like; ALPG active protein
Host
Mammalian cell
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Sequence Positions
20-503aa; Full Length of Mature Protein
Sequence
IIPVEEENPDFWNRQAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTD$
Species
Homo sapiens (Human)
Tag
C-terminal 10xHis-tagged
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Research Area
Cancer
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2 ug/mL can bind Anti-ALPG recombinant antibody, the EC50 is 14.09-23.17 ng/mL.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2?g/mL can bind Anti-ALPG recombinant antibody, the EC50 is 14.09-23.17 ng/mL.)
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2?g/mL can bind Anti-ALPG recombinant antibody, the EC50 is 14.09-23.17 ng/mL.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
Related Product Information for ALPG active protein
May hydrolyze various phosphate compounds.
Product Categories/Family for ALPG active protein
References
"Mutation of a single amino acid converts germ cell alkaline phosphatase to placental alkaline phosphatase."Watanabe T., Wada N., Kim E.E., Wyckoff H.W., Chou J.Y.J. Biol. Chem. 266:21174-21178(1991)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,377 Da
NCBI Official Full Name
alkaline phosphatase, placental-like preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental-like 2
NCBI Official Symbol
ALPPL2
NCBI Official Synonym Symbols
ALPG; GCAP; ALPPL
NCBI Protein Information
alkaline phosphatase, placental-like; ALP-1; Nagao isozyme; germ cell alkaline phosphatase; alkaline phosphatase Nagao isozyme; placental-like alkaline phosphatase; testicular and thymus alkaline phosphatase
UniProt Protein Name
Alkaline phosphatase, placental-like
UniProt Gene Name
ALPPL2
UniProt Synonym Gene Names
ALPPL; GCAP; PLAP-like
UniProt Entry Name
PPBN_HUMAN
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Product Notes
The ALPG alppl2 (Catalog #AAA279192) is an Active Protein produced from Mammalian cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 20-503aa; Full Length of Mature Protein. The amino acid sequence is listed below: IIPVEEENPD FWNRQAAEAL GAAKKLQPAQ TAAKNLIIFL GDGMGVSTVT AARILKGQKK DKLGPETFLA MDRFPYVALS KTYSVDKHVP DSGATATAYL CGVKGNFQTI GLSAAARFNQ CNTTRGNEVI SVMNRAKKAG KSVGVVTTTR VQHASPAGAY AHTVNRNWYS DADVPASARQ EGCQDIATQL ISNMDIDVIL GGGRKYMFPM GTPDPEYPDD YSQGGTRLDG KNLVQEWLAK HQGARYVWNR TELLQASLDP SVTHLMGLFE PGDMKYEIHR DSTLDPSLME MTEAALLLLS RNPRGFFLFV EGGRIDHGHH ESRAYRALTE TIMFDDAIER AGQLTSEEDT LSLVTADHSH VFSFGGYPLR GSSIFGLAPG KARDRKAYTV LLYGNGPGYV LKDGARPDVT ESESGSPEYR QQSAVPLDGE THAGEDVAVF ARGPQAHLVH GVQEQTFIAH VMAFAACLEP YTACDLAPRA GTTD$. It is sometimes possible for the material contained within the vial of "Alkaline phosphatase, germ cell type (ALPG), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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