MEK2 recombinant protein

MEK2, Unactive recombinant protein

Cat. Num. AAA72937

• Gene Names: MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• Applications: Western Blot
• Purity: >90%
• Synonyms: MEK2; N/A; MEK2, Unactive recombinant protein; MAP2K2; MKK2; PRKMK2; MAPKK2; MEK2 recombinant protein

• Purity/Purification: >90%
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.2 ug/ul (varies by lot)
• Sequence Length: 1759

• Applicable Applications for MEK2 recombinant protein: WB (Western Blot), Kinase Assay
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

SDS-PAGE

(The purity of MEK2 was determined to be >90% by densitometry, approx MW ~71kDa)

Related Product Information for MEK2 recombinant protein

Recombinant full-length human MEK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: MEK2 is the member of MAPK kinase (MAPKK) family of signaling protein kinases. MEK2 is a dual-specificity kinase that activates the extracellular signal-regulated kinase (ERK) and mitogen-activated protein (MAP) kinase upon agonist binding to receptors. MEK2 plays a key role in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathways (1). Approximately 30% of all human cancers have a constitutively activated MAPK pathway, and constitutive activation of MEK2 results in cellular transformation. The ERK/MAP kinase cascade regulates cell growth and differentiation (2).

Product Categories/Family for MEK2 recombinant protein

Unactive Kinase; Signaling Proteins - Unactive Kinases

References

1. Shuichan, Xu et al: Differential Regulation of Mitogen-Activated Protein/ERK Kinase (MEK)1 and MEK2 and Activation by a Ras-Independent Mechanism. Mol. Endocrinol. 1997;11 (11): 1618-1625.
2. Louis-Frana§ois, B, et al: Mek2 Is Dispensable for Mouse Growth and Development. Mol. Cellular Biol. 2003; 23 (14): 4778-4787.

NCBI and Uniprot Product Information

• NCBI GI #: 187171274
• NCBI GeneID: 5605
• NCBI Accession #: NP_109587.1
• NCBI GenBank Nucleotide #: NM_030662
• UniProt Accession #: P36507
• Molecular Weight: ~71 kDa
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2), mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: MAP2K2
• NCBI Official Synonym Symbols: CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2; MAPK/ERK kinase 2; MAP kinase kinase 2; ERK activator kinase 2; mitogen-activated protein kinase kinase 2, p45
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• UniProt Gene Name: MAP2K2
• UniProt Synonym Gene Names: MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2
• UniProt Entry Name: MP2K2_HUMAN