Rabbit MEK2 Polyclonal Antibody | anti-MEK2 antibody

MEK2 Antibody (N-term)

Cat. Num. AAA296694

• Gene Names: MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• Reactivity: Human; Predicted Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MEK2, Antibody; MEK2 Antibody (N-term); Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2; MAP2K2; MEK2; MKK2; PRKMK2; anti-MEK2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This MEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MEK2.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

• Applicable Applications for anti-MEK2 antibody: WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence)
• Antigen Region: 1-30
• Cellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane localization is probably regulated by its interaction with KSR1.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Dilution: IHC-P: 1:50~100)

WB (Western Blot)

(Dilution: WB 1:1000Western blot analysis of anti-MEK2 Pab (Cat. #AAA296694) in jurkat cell lysate. MEK2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

IF (Immunofluorescence)

(Dilution: IF 1:10~50Fluorescent image of Hela cell stained with MEK2 Antibody (N-term)(Cat#AAA296694/SG020814R).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). MEK2 immunoreactivity is localized to Cytoplasm significantly.)

Related Product Information for anti-MEK2 antibody

MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.

Product Categories/Family for anti-MEK2 antibody

Primary Antibodies; Cancer; Signal Transduction; Anti-Kinome Antibodies

References

Burroughs, K.D., et al., Mol. Cancer Res. 1 (4):312-322 (2003).Tran, H., et al., Mol. Cell. Biol. 23 (20):7177-7188 (2003).Li, S.P., et al., Cancer Res. 63 (13):3473-3477 (2003).Li, Y., et al., J. Biol. Chem. 278 (16):13663-13671 (2003).Liu, X., et al., J. Biol. Chem. 277 (42):39312-39319 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 13489054
• NCBI GeneID: 5605
• NCBI Accession #: NP_109587.1
• NCBI GenBank Nucleotide #: NM_030662.3
• UniProt Accession #: P36507; P36506; Q63932
• Molecular Weight: 400
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: MAP2K2
• NCBI Official Synonym Symbols: CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2; MAPK/ERK kinase 2; MAP kinase kinase 2; ERK activator kinase 2; mitogen-activated protein kinase kinase 2, p45
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• UniProt Gene Name: MAP2K2
• UniProt Synonym Gene Names: MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2
• UniProt Entry Name: MP2K2_HUMAN