Human Matrix metalloproteinase 14 ELISA Kit | MMP14 elisa kit

Human Matrix metalloproteinase 14 ELISA Kit

Cat. Num. AAA246579

• Gene Names: MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
• Reactivity: Human
• Synonyms: Matrix metalloproteinase 14; N/A; Human Matrix metalloproteinase 14 ELISA Kit; MMP14 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MMP14. No significant cross-reactivity or interference between MMP14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP14 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for MMP14 elisa kit

Principle of the Assay: MMP14 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MMP14 antibody and an MMP14-HRP conjugate. The assay sample and buffer are incubated together with MMP14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP14 concentration since MMP14 from samples and MMP14-HRP conjugate compete for the anti-MMP14 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP14 from the sample, fewer sites are left to bind MMP14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP14 concentration in each sample is interpolated from this standard curve.

Intended Uses: This MMP14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MMP14. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for MMP14 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4826834
• NCBI GeneID: 4323
• NCBI Accession #: NP_004986.1
• NCBI GenBank Nucleotide #: NM_004995.3
• Molecular Weight: 65,894 Da
• NCBI Official Full Name: matrix metalloproteinase-14 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 14 (membrane-inserted)
• NCBI Official Symbol: MMP14
• NCBI Official Synonym Symbols: MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
• NCBI Protein Information: matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase
• UniProt Protein Name: Matrix metalloproteinase-14
• UniProt Gene Name: MMP14
• UniProt Synonym Gene Names: MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP
• UniProt Entry Name: MMP14_HUMAN