Mouse MMP-2 ELISA Kit | MMP2 elisa kit

Mouse MMP-2 PicoKine ELISA Kit

Cat. Num. AAA45276

• Gene Names: Mmp2; GelA; Clg4a; MMP-2
• Reactivity: Mouse
• Synonyms: MMP-2; N/A; Mouse MMP-2 PicoKine ELISA Kit; 72 kDa type IV collagenase; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); 72 kDa gelatinase; CLG 4; CLG 4A; CLG4; CLG4A; Collagenase type IV A; EC 3.4.24.24; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix metalloproteinase 2; Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase); Matrix metalloproteinase II; Matrix metalloproteinase-2; MMP 2; MMP II; MMP2; MMP2_HUMAN; MONA; Neutrophil gelatinase; PEX; TBE 1; TBE-1; MMP2 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse total MMP-2
• Sequence Length: 662
• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml

• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Mouse MMP-2 PicoKine ELISA Kit standard curve)

Related Product Information for MMP2 elisa kit

Principle of the assay: mouse MMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MMP-2 has been precoated onto 96-well plates. Standards (NSO,A30- C662) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MMP-2 amount of sample captured in plate.
Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant mouse MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 461766
• NCBI GeneID: 17390
• UniProt Accession #: P33434
• Molecular Weight: 65,883 Da
• NCBI Official Full Name: 72 kDa type IV collagenase
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: Mmp2
• NCBI Official Synonym Symbols: GelA; Clg4a; MMP-2
• NCBI Protein Information: 72 kDa type IV collagenase; gelatinase A; 72kD gelatinase; 72kDa gelatinase; 72 kDa gelatinase; 72kD type IV collagenase; 72kDa type IV collagenase; matrix metalloproteinase 2; matrix metalloproteinase-2
• UniProt Protein Name: 72 kDa type IV collagenase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: MMP-2
• UniProt Entry Name: MMP2_MOUSE