Rat Myelin-Associated Oligodendrocyte Basic Protein ELISA Kit | MOBP elisa kit

Rat Myelin-Associated Oligodendrocyte Basic Protein ELISA Kit

Cat. Num. AAA247809

• Reactivity: Rat
• Applications: ELISA
• Synonyms: Myelin-Associated Oligodendrocyte Basic Protein; N/A; Rat Myelin-Associated Oligodendrocyte Basic Protein ELISA Kit; MOBP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of MOBP. No significant cross-reactivity or interference between MOBP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MOBP and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Applicable Applications for MOBP elisa kit: ELISA
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for MOBP elisa kit

Principle of the Assay: MOBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MOBP antibody and an MOBP-HRP conjugate. The assay sample and buffer are incubated together with MOBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MOBP concentration since MOBP from samples and MOBP-HRP conjugate compete for the anti-MOBP antibody binding site. Since the number of sites is limited, as more sites are occupied by MOBP from the sample, fewer sites are left to bind MOBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MOBP concentration in each sample is interpolated from this standard curve.

Intended Uses: This MOBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MOBP. This ELISA kit for research use only, not for therapeutic or test applications!