Reactivity
Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of C3d. No significant cross-reactivity or interference between C3d and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C3d and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ug/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for C3d elisa kit
Principle of the Assay: C3d ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C3d antibody and an C3d-HRP conjugate. The assay sample and buffer are incubated together with C3d-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3d concentration since C3d from samples and C3d-HRP conjugate compete for the anti-C3d antibody binding site. Since the number of sites is limited, as more sites are occupied by C3d from the sample, fewer sites are left to bind C3d-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3d concentration in each sample is interpolated from this standard curve.
Intended Uses: This C3d ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey C3d. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This C3d ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey C3d. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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