Reactivity
Mouse
Sequence Length
688
Assay Type
Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for C3d elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse C3d monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
76,684 Da
NCBI Official Full Name
Complement C1s subcomponent
NCBI Official Synonym Full Names
complement component 1, s subcomponent
NCBI Official Symbol
C1S
NCBI Protein Information
complement C1s subcomponent; C1 esterase; basic proline-rich peptide IB-1; complement component 1 subcomponent s
UniProt Protein Name
Complement C1s subcomponent
UniProt Gene Name
C1S
UniProt Entry Name
C1S_HUMAN