NGF/NGF Beta ELISA Kit | NGF elisa kit

Monkey primate NGF/NGF Beta PicoKine ELISA Kit

Cat. Num. AAA124780

• Gene Names: NGF; NGFB; HSAN5; Beta-NGF
• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: NGF/NGF Beta; N/A; Monkey primate NGF/NGF Beta PicoKine ELISA Kit; Beta-nerve growth factor; Beta-NGF; NGF; NGFB; NGF elisa kit

• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant 215 Monkey primate NGF/NGF Beta
• Sequence Length: 241
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <1pg/ml

• Applicable Applications for NGF elisa kit: ELISA
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

Related Product Information for NGF elisa kit

Principle of the Assay: 215 Monkey primate NGF/NGF Beta ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey primate NGF/NGF Beta has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: S122-A241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey primate NGF/NGF Beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Monkey primate NGF/NGF Beta amount of sample captured in plate.

Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male monkey primate submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is monkey primate 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.

Product Categories/Family for NGF elisa kit

Neuroscience; Neurology Process; Growth and Development; neurotrophins; Stem Cells; Neural Stem Cells; Surface Molecules; mesenchymal stem cells

NCBI and Uniprot Product Information

• NCBI GI #: 70995319
• NCBI GeneID: 4803
• NCBI Accession #: NP_002497.2
• NCBI GenBank Nucleotide #: NM_002506.2
• UniProt Accession #: P01138; Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5
• Molecular Weight: 26959 MW
• NCBI Official Full Name: beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: NGF
• NCBI Official Synonym Symbols: NGFB; HSAN5; Beta-NGF
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• UniProt Gene Name: NGF
• UniProt Synonym Gene Names: NGFB; Beta-NGF