Mouse anti-Human 14-3-3 beta Monoclonal Antibody | anti-YWHAB antibody

Anti-14-3-3 beta Antibody, Mouse Monoclonal

Cat. Num. AAA255220

• Gene Names: YWHAB; HS1; GW128; YWHAA; KCIP-1
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Protein A
• Synonyms: 14-3-3 beta, Antibody; Anti-14-3-3 beta Antibody, Mouse Monoclonal; 14-3-3 beta/YWHAB Antibody, Mouse MAb; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta; Anti-GW128 Antibody; Anti-HEL-S-1 Antibody; Anti-HS1 Antibody; Anti-KCIP-1 Antibody; Anti-YWHAA Antibody; anti-YWHAB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 03
• Specificity: Human 14-3-3 beta/YWHAB.; No cross-reactivity in ELISA with E Coli cell lysate (BL21 cell line); Has cross-reactivity in ELISA with Cynomolgus YWHAB
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-YWHAB antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA
• Immunogen: Recombinant Human 14-3-3 beta/YWHAB protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 beta/YWHAB (rh 14-3-3 beta/YWHAB). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Human YWHAB expression on Molt-4 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

IF (Immunofluorescence)

(Immunofluorescence staining of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Related Product Information for anti-YWHAB antibody

14-3-3 beta/YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta/YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta/YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta/YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta/YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.

Product Categories/Family for anti-YWHAB antibody

phospho-Serine/phospho-Threonine Binding Proteins Adaptor Proteins Apoptosis Adaptor Proteins; Death Receptor Signaling

References

Tommerup N, et al. (1996) Assignment of the human genes encoding 14, 3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.

NCBI and Uniprot Product Information

• NCBI GI #: 4507949
• NCBI GeneID: 7529
• NCBI Accession #: NP_003395.1
• NCBI GenBank Nucleotide #: NM_003404.3
• UniProt Accession #: P31946; A8K9K2; E1P616
• Molecular Weight: 28,082 Da
• NCBI Official Full Name: 14-3-3 protein beta/alpha
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
• NCBI Official Symbol: YWHAB
• NCBI Official Synonym Symbols: HS1; GW128; YWHAA; KCIP-1
• NCBI Protein Information: 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptid
• UniProt Protein Name: 14-3-3 protein beta/alpha
• UniProt Gene Name: YWHAB
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433B_HUMAN