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ELISA
(Detection limit for recombinant GST tagged AATF is 0.03 ng/ml as a capture antibody.)
Mouse anti-Human AATF Monoclonal Antibody | anti-AATF antibody
Anti-AATF Antibody (clone 3C7)
Gene Names
AATF; DED; BFR2; CHE1; CHE-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from ascites by Protein A
Synonyms
AATF, Antibody; Anti-AATF Antibody (clone 3C7); Rabbit Polyclonal (IgG) to Human AATF; AATF; CHE1; DED; Rb-binding protein Che-1; CHE-1; Protein AATF; anti-AATF antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
3C7
Specificity
Human AATF
Purity/Purification
Purified from ascites by Protein A
Form/Format
PBS, pH 7.4.
Sequence Length
560
Applicable Applications for anti-AATF antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA
Immunogen
AATF (AAH00591, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C or lower.
Aliquot to avoid repeated freezing and thawing.
Aliquot to avoid repeated freezing and thawing.
ELISA
(Detection limit for recombinant GST tagged AATF is 0.03 ng/ml as a capture antibody.)
ELISA
(Detection limit for recombinant GST tagged AATF is 0.03 ng/ml as a capture antibody.)
WB (Western Blot)
(AATF monoclonal antibody (M04), clone 3C7 Western blot of AATF expression in HeLa NE.)
WB (Western Blot)
(AATF monoclonal antibody (M04), clone 3C7 Western blot of AATF expression in HeLa NE.)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry)
(Anti-AATF antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-AATF antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Related Product Information for anti-AATF antibody
May function as a general inhibitor of the histone deacetylase HDAC1.Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2Fcomplexes, leading to activation of E2F target genes and cell cycleprogression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression.
Product Categories/Family for anti-AATF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,133 Da
NCBI Official Full Name
protein AATF
NCBI Official Synonym Full Names
apoptosis antagonizing transcription factor
NCBI Official Symbol
AATF
NCBI Official Synonym Symbols
DED; BFR2; CHE1; CHE-1
NCBI Protein Information
protein AATF
UniProt Protein Name
Protein AATF
UniProt Gene Name
AATF
UniProt Synonym Gene Names
CHE1; DED
Similar Products
Product Notes
The AATF aatf (Catalog #AAA162294) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-AATF Antibody (clone 3C7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AATF can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the AATF aatf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AATF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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