ACAA2 recombinant antibody

ACAA2 Recombinant Monoclonal Antibody

Cat. Num. AAA278989

• Gene Names: ACAA2; DSAEC
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity purified
• Synonyms: ACAA2, Recombinant Antibody; ACAA2 Recombinant Monoclonal Antibody; 3-ketoacyl-CoA thiolase; mitochondrial (EC 2.3.1.16)(Acetyl-CoA acyltransferase)(Beta-ketothiolase)(Mitochondrial 3-oxoacyl-CoA thiolase)(T1); ACAA2; ACAA2 recombinant antibody

• Host: HEK293F Cell
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 12F4
• Purity/Purification: Affinity purified
• Form/Format: Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.

• Applicable Applications for ACAA2 recombinant antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen Species: Human
• Immunogen: A synthesized peptide derived from Human ACAA2
• Conjugation: Non-conjugated
• Research Area: Cardiovascular; Tags & Cell Markers; Metabolism; Signal transduction
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

IF (Immunofluorescence)

(Immunofluorescence staining of Hela cell with AAA278989 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 570-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

IHC (Immunohiostchemistry)

(IHC image of AAA278989 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

WB (Western Blot)

(Western BlotPositive WB detected in: MCF-7 whole cell lysate, A549 whole cell lysate, Mouse thymusAll lanes: ACAA2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 kDaObserved band size: 42 kDa)

Related Product Information for ACAA2 recombinant antibody

In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs (PubMed:25478839). Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable). Abolishes BNIP3-mediated apoptosis and mitochondrial damage (PubMed:18371312). {ECO:0000269|PubMed:18371312, ECO:0000269|PubMed:25478839, ECO:0000305|PubMed:25478839}.

NCBI and Uniprot Product Information

• NCBI GI #: 167614485
• NCBI GeneID: 10449
• NCBI Accession #: NP_006102.2
• NCBI GenBank Nucleotide #: NM_006111.2
• UniProt Accession #: P42765; Q9BUT6
• Molecular Weight: 41,924 Da
• NCBI Official Full Name: 3-ketoacyl-CoA thiolase, mitochondrial
• NCBI Official Synonym Full Names: acetyl-CoA acyltransferase 2
• NCBI Official Symbol: ACAA2
• NCBI Official Synonym Symbols: DSAEC
• NCBI Protein Information: 3-ketoacyl-CoA thiolase, mitochondrial; T1; acetyl-Coenzyme A acyltransferase 2; beta ketothiolase; beta-ketothiolase; mitochondrial 3-oxoacyl-CoA thiolase; mitochondrial 3-oxoacyl-Coenzyme A thiolase
• UniProt Protein Name: 3-ketoacyl-CoA thiolase, mitochondrial
• UniProt Gene Name: ACAA2
• UniProt Entry Name: THIM_HUMAN