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FCM/FACS (Flow Cytometry)
(ACTA1 Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Mouse ACTA1 Monoclonal Antibody | anti-ACTA1 antibody
ACTA1 Antibody
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Synonyms
ACTA1, Antibody; ACTA1 Antibody; Actin; alpha skeletal muscle; Alpha-actin-1; ACTA1; ACTA; anti-ACTA1 antibody
Host
Mouse
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat)
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
337CT30.10.1
Specificity
This ACTA1 monoclonal antibody is generated from mouse immunized with ACTA1 recombinant protein.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
377
Applicable Applications for anti-ACTA1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ACTA1 Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ACTA1 Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(ACTA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(ACTA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ACTA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ACTA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ACTA1 Antibody western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ACTA1 antibody detected the ACTA1 protein (arrow).)
WB (Western Blot)
(ACTA1 Antibody western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ACTA1 antibody detected the ACTA1 protein (arrow).)
Related Product Information for anti-ACTA1 antibody
The product encoded by this gene belongs to the actin
family of proteins, which are highly conserved proteins that play a
role in cell motility, structure and integrity. Alpha, beta and
gamma actin isoforms have been identified, with alpha actins being
a major constituent of the contractile apparatus, while beta and
gamma actins are involved in the regulation of cell motility. This
actin is an alpha actin that is found in skeletal muscle. Mutations
in this gene cause nemaline myopathy type 3, congenital myopathy
with excess of thin myofilaments, congenital myopathy with cores,
and congenital myopathy with fiber-type disproportion, diseases
that lead to muscle fiber defects.
family of proteins, which are highly conserved proteins that play a
role in cell motility, structure and integrity. Alpha, beta and
gamma actin isoforms have been identified, with alpha actins being
a major constituent of the contractile apparatus, while beta and
gamma actins are involved in the regulation of cell motility. This
actin is an alpha actin that is found in skeletal muscle. Mutations
in this gene cause nemaline myopathy type 3, congenital myopathy
with excess of thin myofilaments, congenital myopathy with cores,
and congenital myopathy with fiber-type disproportion, diseases
that lead to muscle fiber defects.
Product Categories/Family for anti-ACTA1 antibody
References
Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) :
Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010)
Yu, G., et al. J Clin Neurosci 17(6):766-769(2010)
Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010) :
Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)
Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010)
Yu, G., et al. J Clin Neurosci 17(6):766-769(2010)
Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010) :
Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42051
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
NCBI Protein Information
actin, alpha skeletal muscle
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
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Product Notes
The ACTA1 acta1 (Catalog #AAA283495) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACTA1 Antibody reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ACTA1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ACTA1 acta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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