IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35))
Mouse Actin Monoclonal Antibody | anti-ACTA1 antibody
Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human, Rabbit, Cat, Dog, Mouse, Rat, Chicken
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
Actin, Antibody; Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody; ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1; anti-ACTA1 antibody
Host
Mouse
Reactivity
Human, Rabbit, Cat, Dog, Mouse, Rat, Chicken
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
HHF35
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
377
Applicable Applications for anti-ACTA1 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Cytoplasmic
Immunogen
SDS extract of human myocardium.
Hu-Chromosome Location
1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
Positive Control
Muscle or sarcoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35))
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35))
References
Tsukada T. et. al. Am J Pathol 1987, 126:51. Tsukada T. et. al. Am J Pathol 1987, 127:389.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,009 Da
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
NCBI Protein Information
actin, aortic smooth muscle
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
UniProt Entry Name
ACTA_HUMAN
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Product Notes
The ACTA1 acta2 (Catalog #AAA62542) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody reacts with Human, Rabbit, Cat, Dog, Mouse, Rat, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Actin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ACTA1 acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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