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WB (Western Blot) (Western BlotPositive WB detected in: 20ug Hela whole cell lysateACTB antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

Mouse anti-Human ACTB Monoclonal Antibody | anti-ACTB antibody

ACTB Monoclonal Antibody

Reactivity
Human
Applications
ELISA, Western Blot
Purity
>95%, Protein G purified
Synonyms
ACTB; Monoclonal Antibody; ACTB Monoclonal Antibody; anti-ACTB antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D8D8
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for anti-ACTB antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:10000-1:1280000
Immunogen
A synthesized peptide derived from Human Beta-Actin (1-50AA)
Conjugation
Non-conjugated
Research Area
Others
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

WB (Western Blot)

(Western BlotPositive WB detected in: 20ug Hela whole cell lysateACTB antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: 20ug Hela whole cell lysateACTB antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot)

(Western BlotPositive WB detected in: Hela whole cell lysate at 40ug, 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ugAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate at 40ug, 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ugAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot)

(Western BlotPositive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit lung tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue, A549 whole cell lysate, MCF-7 whole cell lysateAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit lung tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue, A549 whole cell lysate, MCF-7 whole cell lysateAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot)

(Western BlotPositive WB detected in: Rat heart tissue, Rat liver tissue, Rat kidney tissue, U87 whole cell lysate Rat skeletal muscle tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Rat heart tissue, Rat liver tissue, Rat kidney tissue, U87 whole cell lysate Rat skeletal muscle tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot)

(Western BlotPositive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse skeletal muscle tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse skeletal muscle tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot)

(Western BlotPositive WB detected in: 293T whole cell lysate, Zebrafish tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: 293T whole cell lysate, Zebrafish tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot)

(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue, Rat heart tissue, Rat skeletal muscle tissue, Rabbit lung tissue, Rabbit liver tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue, Rat heart tissue, Rat skeletal muscle tissue, Rabbit lung tissue, Rabbit liver tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

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Product Notes

The ACTB (Catalog #AAA28072) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACTB Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACTB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:10000-1:1280000. Researchers should empirically determine the suitability of the ACTB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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