Rabbit Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) Monoclonal Antibody | anti-ACTB antibody

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

Cat. Num. AAA26910

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Bovine, Human, Monkey, Mouse, Porcine, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin), Antibody; Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin); Actin, beta; anti-ACTB antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Monkey, Mouse, Porcine, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 10g129(13E5)
• Specificity: Recognizes endogenous levels of total human beta-actin protein at ~45kD. May crossreact with the gamma-actin (cytoplasmic isoform). Does not crossreact with alpha-skeletal, alpha-cardiac, alpha-vascular smooth or gamma-enteric smooth muscle isoforms. Species Crossreactivity: mouse, rat, monkey, bovine, porcine.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-ACTB antibody: Flow Cytometry (FC/FACS), Immunochemistry (IC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Immunofluorescence (IF-IC): 1:100. Methanol permeabilization is required.; Flow Cytometry: 1:100; Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:200. Antigen retrieval with citrate buffer is recommended.; Immunohistochemistry (Frozen): 1:200. Fixation in 10% Neutral buffered formalin is recommended.
• Immunogen: Synthetic peptide corresponding to residues near the N-terminal residues of human beta-actin
• Species Sequence Homology: chicken, canine, equine.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma using AAA26910(left) or beta-Actin Blocking Peptide (right).)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using AAA26910.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of COS-7 cells using AAA26910 (green). Blue pseudocolor= DRAQ5 fluorescent DNA dye)

FCM (Flow Cytometry)

(Flow cytometric analysis of NIH/3T3 cells using AAA26910 (blue) compared to a nonspecific negative control antibody (red).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human leiomyoma using AAA26910)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human heart using AAA26910. Note the lack of staining of cardiac actin.)

WB (Western Blot)

(Western blot analysis of cell extracts from various cell lines using AAA26910.)

Related Product Information for anti-ACTB antibody

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle b- and g-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). a-cardiac anda-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, a- and g-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6).

Product Categories/Family for anti-ACTB antibody

Antibodies-Primary; Antibodies-Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4501885
• NCBI GeneID: 60
• NCBI Accession #: NP_001092.1
• NCBI GenBank Nucleotide #: NM_001101.3
• UniProt Accession #: P60709; P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
• Molecular Weight: 375
• NCBI Official Full Name: actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin, beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
• UniProt Protein Name: Actin, cytoplasmic 1
• UniProt Gene Name: ACTB
• UniProt Entry Name: ACTB_HUMAN