Mouse Actin Monoclonal Antibody | anti-ACTA2 antibody

Actin, Smooth Muscle (Leiomyosarcoma Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62894

• Gene Names: ACTA2; AAT6; ACTSA; MYMY5
• Reactivity: Human, Rat.; Predicted to show a broad reactivity
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: Actin, Antibody; Actin, Smooth Muscle (Leiomyosarcoma Marker) Mouse Monoclonal Antibody; ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46; anti-ACTA2 antibody

• Host: Mouse
• Reactivity: Human, Rat.; Predicted to show a broad reactivity
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: ACTA2/791
• Specificity: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 377

• Applicable Applications for anti-ACTA2 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cytoplasmic
• Immunogen: Recombinant full-length human ACTA2 protein
• Hu-Chromosome Location: 10q23.31
• Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Lung stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).)

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).)

References

Gown, A.M., et al. 1985. A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes. J. Cell Biol. 100: 807-813.

NCBI and Uniprot Product Information

• NCBI GI #: 213688375
• NCBI GeneID: 59
• NCBI Accession #: NP_001135417.1
• NCBI GenBank Nucleotide #: NM_001141945.2
• UniProt Accession #: P62736; P03996; P04108; Q6FI19; B2R8A4
• Molecular Weight: 42kDa
• NCBI Official Full Name: actin, aortic smooth muscle
• NCBI Official Synonym Full Names: actin, alpha 2, smooth muscle, aorta
• NCBI Official Symbol: ACTA2
• NCBI Official Synonym Symbols: AAT6; ACTSA; MYMY5
• NCBI Protein Information: actin, aortic smooth muscle
• UniProt Protein Name: Actin, aortic smooth muscle
• UniProt Gene Name: ACTA2
• UniProt Synonym Gene Names: ACTSA; ACTVS
• UniProt Entry Name: ACTA_HUMAN