IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Monoclonal Antibody (SPM334).)
Mouse AFP (Alpha Fetoprotein) Monoclonal Antibody | anti-AFP antibody
AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Mouse Monoclonal Antibody
Gene Names
AFP; AFPD; FETA; HPAFP
Reactivity
Human, Monkey, Dog, Pig.Does not react with Cow, Cat, Mouse, and Rat
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
AFP (Alpha Fetoprotein), Antibody; AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Mouse Monoclonal Antibody; Alpha fetoglobulin; FETA; HPAFP; anti-AFP antibody
Host
Mouse
Reactivity
Human, Monkey, Dog, Pig.
Does not react with Cow, Cat, Mouse, and Rat
Does not react with Cow, Cat, Mouse, and Rat
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
SPM334
Specificity
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
609
Applicable Applications for anti-AFP antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Cytoplasmic
Immunogen
Alpha fetoprotein (AFP) purified from serum of a hepatoma patient
Hu-Chromosome Location
4q13.3
Positive Control
Hep-G2 cells. Fetal liver or hepatocellular carcinoma
Preparation and Storage
Antibody with azide - store at 2 to 8°C
Antibody without azide - store at -20 to -80°C.
Antibody without azide - store at -20 to -80°C.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Monoclonal Antibody (SPM334).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Monoclonal Antibody (SPM334).)
References
Yazova AK; Goussev AI; Poltoranina VS; Yakimenko EF. Human alpha-fetoprotein epitopes as revealed by monoclonal antibodies. Immunology Letters, 1990 Sep, 25(4):325-30
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70kDa
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
AFPD; FETA; HPAFP
NCBI Protein Information
alpha-fetoprotein
UniProt Protein Name
Alpha-fetoprotein
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
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Product Notes
The AFP afp (Catalog #AAA62502) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Mouse Monoclonal Antibody reacts with Human, Monkey, Dog, Pig. Does not react with Cow, Cat, Mouse, and Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AFP (Alpha Fetoprotein) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the AFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFP (Alpha Fetoprotein), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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