Mouse Aggrecan, N-terminal neoepitope DIPEN Monoclonal Antibody | anti-ACAN antibody
Monocloncal Antibody DIPEN (Clone BC4)
Gene Names
ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity purified on protein G
Synonyms
Aggrecan, N-terminal neoepitope DIPEN, Antibody; Monocloncal Antibody DIPEN (Clone BC4); Aggrecan Antibody, N-terminal neoepitope DIPEN; anti-ACAN antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
BC-4
Specificity
Recognizes the N-terminal neoepitope sequence (...DIPEN) generated at the “MMP cleavage site” after MMP catabolism in the interglobular domain of aggrecan between amino acids ..PEN341 and 342FFG.
Purity/Purification
Affinity purified on protein G
Form/Format
Liquid; PBS, no preservatives
Concentration
0.1 mg/mL (varies by lot)
Sequence Length
721
Applicable Applications for anti-ACAN antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA
Application Notes
Western Blot: 1:100
Detects a band of approximately 60 kDa
Immunohistochemistry:
This antibody should work in IHC on formalin-or paraformaldehyde-fixed paraffin embedded sections as well as either alcochol-fixed frozen sections or un-fixed snap-frozen sections.
Detects a band of approximately 60 kDa
Immunohistochemistry:
This antibody should work in IHC on formalin-or paraformaldehyde-fixed paraffin embedded sections as well as either alcochol-fixed frozen sections or un-fixed snap-frozen sections.
Immunogen
DIPEN synthetic peptide conjugate.
Myeloma
x63-Ag8.653
Light Chain Type
kappa
Cross Reactivity
Human, Rat, Guinea Pig, Horse, Pig.
Technical Notes
Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry (1, 2).
Preparation and Storage
Store at -20°C. Repeated freezing and thawing should be avoided. Aliquoting is recommended.
Related Product Information for anti-ACAN antibody
This metabolite usually remains in the tissue complexed to hyaluronan but with extensive cartilage catabolism it may be released from the tissue.
Product Categories/Family for anti-ACAN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Aggrecan
NCBI Official Synonym Full Names
aggrecan
NCBI Official Symbol
ACAN
NCBI Official Synonym Symbols
AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
NCBI Protein Information
aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1
UniProt Protein Name
Aggrecan core protein
UniProt Gene Name
ACAN
UniProt Synonym Gene Names
AGC1; CSPG1; MSK16; CSPCP
UniProt Entry Name
PGCA_HUMAN
Similar Products
Product Notes
The ACAN acan (Catalog #AAA13857) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Aggrecan, N-terminal neoepitope DIPEN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA. Western Blot: 1:100 Detects a band of approximately 60 kDa Immunohistochemistry: This antibody should work in IHC on formalin-or paraformaldehyde-fixed paraffin embedded sections as well as either alcochol-fixed frozen sections or un-fixed snap-frozen sections. Researchers should empirically determine the suitability of the ACAN acan for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aggrecan, N-terminal neoepitope DIPEN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.