IF (Immunofluorescence)
(ICC/IF analysis of AKR1C1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AKR1C1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human AKR1C1 Monoclonal Antibody | anti-AKR1C1 antibody
AKR1C1 antibody
Gene Names
AKR1C1; C9; DD1; DDH; DDH1; H-37; HBAB; MBAB; HAKRC; DD1/DD2; 2-ALPHA-HSD; 20-ALPHA-HSD
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
AKR1C1, Antibody; AKR1C1 antibody; Aldo-keto reductase family 1 member C1; 20-alpha-HSD; DD1/DD2; HBAB; DDH; DDH1; Aldo-keto reductase family 1; member C1 20 alpha (3 alpha) hydroxysteroid dehydrogenase; 20 alpha hydroxysteroid dehydrogenase; 2ALPHAHSD; AK1C1; MBAB; Aldo keto reductase family 1 member C1; C9; Chlordecone reductase homolog; Chlordecone reductase homolog HAKRC; DD1; Dihydrodiol dehydrogenase 1; Dihydrodiol dehydrogenase 1/2; H37; HAKRC; Hepatic dihydrodiol dehydrogenase; High affinity hepatic bile acid-binding protein; Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase; Type II 3 alpha hydroxysteroid dehydrogenase,; anti-AKR1C1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT6D10
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
323
Applicable Applications for anti-AKR1C1 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human AKR1C1 (1-323aa) purified from E. coli
Clone Note
Anti-human AKR1C1 mAb, clone AT6D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AKR1C1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of AKR1C1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AKR1C1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of AKR1C1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AKR1C1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AKR1C1 Lane 2. : HepG2 cell lysate Lane 3. : Raji cell lysate)
WB (Western Blot)
(The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AKR1C1 Lane 2. : HepG2 cell lysate Lane 3. : Raji cell lysate)
Related Product Information for anti-AKR1C1 antibody
The human aldo-keto reductases 1C1 and 1C3 (AKR1C1 and AKR1C3) have major roles in pre receptor regulation of progesterone action. They can both convert progesterone to the less potent efficiencies. AKR1C1 and AKR1C3 also act as 3-ketosteroid reductase, and as such they can convert the most potent androgen 5alpha-DHT into 3beta-andorstandiol, which is an estrogen receptor beta ligand, and into the inactive androgen 3alpha-androstnionl, respectively.
Product Categories/Family for anti-AKR1C1 antibody
References
L.C. Giudice, et al. (2004) Lancet. 3644: 1789-1799; P.G. Hompes, et al. (2007) Gynecol. Endocrinol. 23: 5-12.; K.J. Berkley, et al. (2005) Science 308: 1587-1589.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,788 Da
NCBI Official Full Name
aldo-keto reductase family 1 member C1
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member C1
NCBI Official Symbol
AKR1C1
NCBI Official Synonym Symbols
C9; DD1; DDH; DDH1; H-37; HBAB; MBAB; HAKRC; DD1/DD2; 2-ALPHA-HSD; 20-ALPHA-HSD
NCBI Protein Information
aldo-keto reductase family 1 member C1
UniProt Protein Name
Aldo-keto reductase family 1 member C1
UniProt Gene Name
AKR1C1
UniProt Synonym Gene Names
DDH; DDH1; 20-alpha-HSD; DD1/DD2; HBAB
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Product Notes
The AKR1C1 akr1c1 (Catalog #AAA48091) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AKR1C1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKR1C1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the AKR1C1 akr1c1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKR1C1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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