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Mouse AKT1 Monoclonal Antibody | anti-AKT1 antibody
AKT1 (v-akt murine Thymoma Viral Oncogene Homolog 1, AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA) (PE)
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Applications
Immunofluorescence, Western Blot
Purity
Purified
Synonyms
AKT1, Antibody; AKT1 (v-akt murine Thymoma Viral Oncogene Homolog 1, AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA) (PE); v-akt murine Thymoma Viral Oncogene Homolog 1; AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; anti-AKT1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1D4
Specificity
Recognizes AKT1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Sequence Length
480
Applicable Applications for anti-AKT1 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
AKT1 (AAH00479.1, 1aa-480aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTLHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
WB (Western Blot)
(AKT1 monoclonal antibody (M08), clone 1D4. Western Blot analysis of AKT1 expression in Raw 264.7.)
WB (Western Blot)
(AKT1 monoclonal antibody (M08), clone 1D4. Western Blot analysis of AKT1 expression in NIH/3T3.)
Application Data
(Detection limit for recombinant GST tagged AKT1 is approximately 1ng/ml as a capture antibody.)
WB (Western Blot)
(AKT1 monoclonal antibody (M08), clone 1D4 Western Blot analysis of AKT1 expression in Jurkat (Cat # L017V1).)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml])
Related Product Information for anti-AKT1 antibody
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
Product Categories/Family for anti-AKT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
V-akt murine thymoma viral oncogene homolog 1
NCBI Official Synonym Full Names
AKT serine/threonine kinase 1
NCBI Official Symbol
AKT1
NCBI Official Synonym Symbols
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
NCBI Protein Information
RAC-alpha serine/threonine-protein kinase
